Difference between revisions of "Team:KAIT Japan/Protocol"
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| − | </ | + | <strong>[SDS-PAGE]</strong><br> |
| + | 1) Prepare the gel<br> | ||
| + | 2) Place stacking gel on separating gel <br> | ||
| + | 3) Fill electrophoresis chamber electrophoretic buffer<br> | ||
| + | 4) Add 4×sample buffer the cooldshock expressed protein sample and heat block (95℃,10minutes) <br> | ||
| + | 5) Load prepared sample into wells <br> | ||
| + | 6) Run the electrophoresis in 15mA, 1 hour 25 minutes<br> | ||
| + | 7) Dye the gel with CBB for 30 minutes<br> | ||
| + | 8) Branch the gel for 2 hours while shaking it<br> | ||
| + | <br> | ||
| + | <img src=""> | ||
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| + | </font> | ||
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Revision as of 07:16, 18 September 2015
Protocol
[SDS-PAGE]
1) Prepare the gel
2) Place stacking gel on separating gel
3) Fill electrophoresis chamber electrophoretic buffer
4) Add 4×sample buffer the cooldshock expressed protein sample and heat block (95℃,10minutes)
5) Load prepared sample into wells
6) Run the electrophoresis in 15mA, 1 hour 25 minutes
7) Dye the gel with CBB for 30 minutes
8) Branch the gel for 2 hours while shaking it
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