Difference between revisions of "Team:KAIT Japan/Protocol"

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Revision as of 07:26, 18 September 2015

Protocol


[SDS-PAGE]
1) Prepare the gel
2) Place stacking gel on separating gel
3) Fill electrophoresis chamber electrophoretic buffer
4) Add 4×sample buffer the cooldshock expressed protein sample and heat block (95℃,10minutes)
5) Load prepared sample into wells
6) Run the electrophoresis in 15mA, 1 hour 25 minutes
7) Dye the gel with CBB for 30 minutes
8) Branch the gel for 2 hours while shaking it







[Native-PAGE]
1) Prepare the gel
2) Place stacking gel on separating gel
3) Fill electrophoresis chamber electrophoretic buffer
4) Add 3×sample buffer
5) Load prepared sample into wells
6) Run the electrophoresis in 15mA, 1 hour 25 minutes
7) Dye the gel with CBB for 30 minutes
8) Branch the gel for 2 hours while shaking it