Difference between revisions of "Team:Pasteur Paris/Week 10"
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<li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li></br> | <li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li></br> | ||
<li style="text-align:left">Solubilization of TPA by NaOH</li></br> | <li style="text-align:left">Solubilization of TPA by NaOH</li></br> | ||
− | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0, | + | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5 mol/l</li></br> |
<li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | ||
<li style="text-align:left"><u>Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:</u></br>Media 1 to 10 are made in solid form: | <li style="text-align:left"><u>Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:</u></br>Media 1 to 10 are made in solid form: |
Revision as of 12:55, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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