Difference between revisions of "Team:KAIT Japan/Notebook"

 
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6/22~7/24:PCR(Dronpa145N,Dronpa145K)<br>
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Please look at this <a href="https://static.igem.org/mediawiki/2015/1/1c/Lab_note22.pdf">Link</a>.
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7/25~7/28:Digested PCR Product of Dronpa 145N,K(6/22) and pCold Vector<br>
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7/29~7/31:Ligation (Dronpa145N +pCold Vector),and transformation the ligation product.<br>
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8/1:Get three clonies that brite under UV light, pick up these colonies and incubate in liquide culuture<br>
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8/2:Miniprep the clones and digest plasmids to confirm we get correct clones<br>
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8/3:purified plasmid(Dronpa 145N+pCold)<br>
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8/4:transform RFP to Ecoli<br>
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8/5:Glycerol stock clones that have plasmid(Dronpa 145N+pcold)<br>
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8/6:digested PCR Product of Dronpa 145N,K(7/29) by EcorⅠand PstⅠ<br>
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8/7:After miniprep RFP clone, Digested the plasmid (RFP+pSB1C3) by EcoRⅠand PstⅠ,Ligation (pSB1C3+Dronpa 145N,K),transform the ligation product to Ecoli<br>
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8/8~8/12:incubated clones (pCold+Dronpa145N) to exprese Dronpa 145N<br>
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8/13~8/14:digested plasmid (pCold+Dronpa145N) by EcoRⅠ and XhoⅠ<br>
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8/15~8/18:digested Dronpa 145K by EcorⅠand PstⅠ，inserted into pCold vector by ligase,transform it into Ecoli<br>
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8/19~8/22:ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.<br>
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8/23:digested purifyide plsmid to check insert，we are not able to get good result ,try again<br>
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8/24~8/25:digested  Dronpa145N by EcoRⅠ and, XhoⅠ,ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.<br>
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8/26:colony PCR<br>
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8/27:<br>
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8/28~8/29:<br>
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8/30:<br>
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8/31:<br>
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9/1:<br>
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9/8~9/10:<br>
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<font size="9" face="Freestyle Script">Link list</font>
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<font size="9" face="Freestyle Script">---Link list---</font>
 
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Latest revision as of 15:27, 18 September 2015

Lab note



Please look at this Link.