Difference between revisions of "Team:Czech Republic/Goals"
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*Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | *Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | ||
==Goals of module 2== | ==Goals of module 2== | ||
| − | * Construct a set of yeast plasmids with different mating pheromones and their receptors and | + | * Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks |
* Verify the correct coupling of the receptors to the yeast pheromone mating pathway | * Verify the correct coupling of the receptors to the yeast pheromone mating pathway | ||
* Verify the correct expression and secretion of the different pheromones | * Verify the correct expression and secretion of the different pheromones | ||
Revision as of 18:31, 18 September 2015
Goals
Contents
Golas of module 1
- Construct a set of reporter promoters for yeast cells
- Characterize reporter promoters in all mating types
- Design synthetic MATa and MATx strains
- Build a synthetic diploid strain with a functional yeast pheromone pathway
- Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids
Goals of module 2
- Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks
- Verify the correct coupling of the receptors to the yeast pheromone mating pathway
- Verify the correct expression and secretion of the different pheromones
- Show the orthogonality of the used receptors and pheromones