Difference between revisions of "Team:Czech Republic/Goals"
(→Goals of module 2) |
|||
| Line 7: | Line 7: | ||
*Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | *Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | ||
==Goals of module 2== | ==Goals of module 2== | ||
| − | * | + | * Constructed a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks |
| − | * | + | * Verified the correct coupling of the receptors to the yeast pheromone mating pathway |
| − | * | + | * Verified the correct expression and secretion of the different pheromones |
| − | * | + | * Showed the orthogonality of the used receptors and pheromones |
| + | |||
==Goals of module 3== | ==Goals of module 3== | ||
* Demonstrate the ability of our receptors to bind chosen markers. | * Demonstrate the ability of our receptors to bind chosen markers. | ||
Revision as of 18:40, 18 September 2015
Goals
Golas of module 1
- Construct a set of reporter promoters for yeast cells
- Characterize reporter promoters in all mating types
- Design synthetic MATa and MATx strains
- Build a synthetic diploid strain with a functional yeast pheromone pathway
- Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids
Goals of module 2
- Constructed a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks
- Verified the correct coupling of the receptors to the yeast pheromone mating pathway
- Verified the correct expression and secretion of the different pheromones
- Showed the orthogonality of the used receptors and pheromones
Goals of module 3
- Demonstrate the ability of our receptors to bind chosen markers.