Difference between revisions of "Team:Czech Republic/Goals"
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{{:Team:Czech_Republic/Template:Top|Goals}} | {{:Team:Czech_Republic/Template:Top|Goals}} | ||
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*Construct a set of reporter promoters for yeast cells | *Construct a set of reporter promoters for yeast cells | ||
*Characterize reporter promoters in all mating types | *Characterize reporter promoters in all mating types | ||
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*Build a synthetic diploid strain with a functional yeast pheromone pathway | *Build a synthetic diploid strain with a functional yeast pheromone pathway | ||
*Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | *Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids | ||
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==Goals of module 2== | ==Goals of module 2== | ||
* Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with the BioBricks | * Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with the BioBricks | ||
Revision as of 18:49, 18 September 2015
Goals
Goals of module 1
- Construct a set of reporter promoters for yeast cells
- Characterize reporter promoters in all mating types
- Design synthetic MATa and MATx strains
- Build a synthetic diploid strain with a functional yeast pheromone pathway
- Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids
Goals of module 2
- Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with the BioBricks
- Verify the correct coupling of the receptors to the yeast pheromone mating pathway
- Verify the correct expression and secretion of the different pheromones
- Show the orthogonality of the used receptors and pheromones
Goals of module 3
- Demonstrate the ability of our receptors to bind chosen markers.