Difference between revisions of "Team:Utah State"

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<title>Arcana by HTML5 UP</title>
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<h4> Project Description </h4>
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<p>Bacteria are used for a number of important purposes within the bioprocess and food industries. One notable example is the fermentation of dairy products in order to create cheese and yogurt. One problem that practitioners of bacteria fermentation deal with is attack by bacteriophages—viruses that infect bacteria. Many different techniques are used to control phage infection, including prevention of phage contamination by routine cleaning of the fermentation areas and rotation of cultures, employment of phage resistant strains of bacteria, and efforts to minimize opportunities for the appearance of new strains of phage. With the rise of molecular biotechnology, it is possible to create new types phage resistant bacteria by stacking genes with defense systems into the same strain and genetic construct. The dairy industry has funded research of this sort to creating phage resistant strains of lactic acid bacteria.</p>  
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<p>Building on research aimed at the creation of phage resistant lactic acid bacteria, the 2015 Utah State University iGEM team is using synthetic biology to create a strain of Lactococcus lactis that is capable of detecting, reporting, and fighting against phage infection, specifically the bacteriophage P335 from the Siphoviridae family. These bacteria will work by using a promoter from the virus itself, known as the cro promoter system, which will activate when the phage is present. In a construct where the bacteria will report the presence of phage so the culture can be stopped before the infection spreads, a GFP or RFP that is functional in the L. lactis bacteria will be placed downstream of the promoter. This will allow technicians to measure the fluorescence levels present in the culture to gain an indication of phage presence at any given time. In the version where the bacteria are able to fight against the infection, a suicide system will be placed downstream from the promoter system, which will cause the bacteria to die before the phage is able to fully form, preventing further spread throughout the culture.</p>  
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<p>In addition, another goal for the USU team is to test the viability of promoters and fluorescent proteins that were designed for lactic acid bacteria in L. lactis.</p>  
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<h2><!--<a href="index.html" id="logo">-->Utah State University <em>iGEM 2015</em></a></h2>
  
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<h2> Welcome to iGEM 2015! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<nav id="nav">
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<ul>
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<li><a href="https://2015.igem.org/Team:Utah_State">Home</a></li>
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                                <li><a href="https://2015.igem.org/Team:Utah_State/Team">Team</a></li>
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<li>
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<a href="#">Project</a>
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<ul>
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<li><a href="https://2015.igem.org/Team:Utah_State/Description">Description</a></li>
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<li><a href="https://2015.igem.org/Team:Utah_State/Experiments">Experiments & Protocols</a></li>
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<li><a href="https://2015.igem.org/Team:Utah_State/Results">Results</a></li>
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                                        <li><a href="https://2015.igem.org/Team:Utah_State/Design">Design</a></li>
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<li><a href="#">Magna phasellus</a></li>
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</ul>
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<li><a href="https://2015.igem.org/Team:Utah_State/Interlab">Interlab Study</a></li>
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<li>
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<a href="https://2015.igem.org/Team:Utah_State/Parts">Parts</a>
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<ul>
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<li><a href="https://2015.igem.org/Team:Utah_State/Parts">All Parts</a></li>
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<li><a href="https://2015.igem.org/Team:Utah_State/Basic_Part">Favorite Parts</a></li>
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</ul>
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</li>
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<li><a href="https://2015.igem.org/Team:Utah_State/Attributions">Attributions</a></li>
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                                <li><a href="https://2015.igem.org/Team:Utah_State/Collaborations">Collaborations</a></li>
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                                <li><a href="https://2015.igem.org/Team:Utah_State/Practices">Human Practices</a></li>
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                                <li><a href="https://2015.igem.org/Team:Utah_State/Safety">Safety</a></li>
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                                <li><a href="https://2015.igem.org/Team:Utah_State/Standards">Medal Criteria</a></li>
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</ul>
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<h4>Before you start: </h4>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<div class="highlightBox">
 
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<h4> Styling your wiki </h4>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
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<h4> Editing your wiki </h4>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Utah_State&action=edit"> Click here to edit this page! </a></p>
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<section id="banner" style="background-image:<layer id='placeholderlayer'></layer><div id='placeholderdiv'></div>">  
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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<h2><em>We are programming a phage resistant </em><i>Lactococcus lactis</i><em> to protect your cheese!</em></h2>
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<a href="https://2015.igem.org/Team:Utah_State/Description" class="button">Interested?</a>
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</header>
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</section>
  
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<h4>Templates </h4>
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<div class="row 200%">
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<section class="4u 12u(narrower)">
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<div class="box highlight">
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<i class="icon major fa-paper-plane"></i>
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<h3>This Is Important</h3>
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<h3>Also Important</h3>
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<h3>Probably Important</h3>
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<div class="container">
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<header class="major">
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<h2>Abstract</h2>
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<p style="color: #333333">The cheese industry generates billions of dollars each year, and no wonder—cheese is delicious! One of the greatest difficulties that the cheese industry faces is bacteriophage (virus) infection, which kills bacteria used in the manufacture of cheese. The industry utilizes a variety of approaches to deal with this problem, each having their own drawbacks. The 2015 USU iGEM team is implementing a synthetic biology approach to design and create phage resistant <i>Lactococcus lactis</i>, a commonly-used cheese starter bacteria. The genetic mechanism behind this phage resistance functions by pairing a promoter that is activated in the presence of phage with a kill switch to terminate infected cells before phage are able to propagate. </p>
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</div>
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</section>
  
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<h4>Tips</h4>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<section class="wrapper style1">
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<ul>
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<div class="container">
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<div class="row">
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<section class="6u 12u(narrower)">
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<div class="box post">
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<a href="https://2015.igem.org/Team:Utah_State/Results" class="image left"><img src="https://static.igem.org/mediawiki/2015/f/f7/Team_Utah_State_USUGlopic.jpg" width="100%" alt="" /></a>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<div class="inner">
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<h3><a href="https://2015.igem.org/Team:Utah_State/Results">Results</a></h3>
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<li>Have lots of fun! </li>
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<p>Take a look at what we accomplished.</p>
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</ul>  
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</div>
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</div>
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</section>
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<section class="6u 12u(narrower)">
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<div class="box post">
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<a href="https://2015.igem.org/Team:Utah_State/Parts" class="image left"><img src="https://static.igem.org/mediawiki/2015/8/8f/Team_Utah_State_PartsThumb.jpg" width="100%" ></a><br>
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<div class="inner">
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<h3><a href="https://2015.igem.org/Team:Utah_State/Parts">Parts</a></h3>
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<p>Here are our Biobricks.</p>
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</div>
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</div>
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</section>
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</div>
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<div class="row">
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<section class="6u 12u(narrower)">
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<div class="box post">
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<a href="https://2015.igem.org/Team:Utah_State/Standards" class="image left"><img src="https://static.igem.org/mediawiki/2015/6/61/Team_Utah_State_MedalsSquare.jpg" width="100%" /></a>
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<div class="inner">
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<h3><a href="https://2015.igem.org/Team:Utah_State/Standards">Medal Criteria</a></h3>
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<p>Here's what we did to deserve a gold medal.</p>
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</div>
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</div>
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</section>
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<section class="6u 12u(narrower)">
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<div class="box post">
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<a href="https://2015.igem.org/Team:Utah_State/Team" class="image left"><img src="https://static.igem.org/mediawiki/2015/8/8f/Team_Utah_State_TeamThumb.jpg" width="100%" /></a>
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<div class="inner">
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<h3><a href="https://2015.igem.org/Team:Utah_State/Team">The Team</a></h3>
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<p>Meet the people who made this project possible.</p>
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</div>
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</div>
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</section>
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</div>
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</div>
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</section>
  
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                      <!-- CTA -->
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<!--<section id="cta" class="wrapper style3">
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<div class="container">
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<header>
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<h2>Are you ready to continue your quest?</h2>
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<a href="#" class="button">Insert Coin</a>
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</header>
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</div>
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</section>-->
  
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<h4>Inspiration</h4>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<div class="container">
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<section> <!--class="3u 6u(narrower) 12u$(mobilep)"-->
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<h3 style="text-align: center">Links to Stuff</h3>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<tr>
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<td><a href="https://2015.igem.org/Team:Utah_State">Home</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Team">Team</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Standards">Medal Criteria</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Attributions">Attributions</a></td>
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</tr>
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<tr>
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<td><a href="https://2015.igem.org/Team:Utah_State/Description">Project Description</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Experiments">Experiments and Protocols</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Results">Results</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Design">Design</a></td>
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</tr>
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<td><a href="https://2015.igem.org/Team:Utah_State/Interlab">Interlab Study</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Practices">Human Practices</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Collaborations">Collaborations</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Safety">Safety</a></td>
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<tr>
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<td><a href="https://2015.igem.org/Team:Utah_State/Parts">All Parts</a></td>
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<td><a href="https://2015.igem.org/Team:Utah_State/Basic_Part">Favorite Parts</a></td>
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</tr>
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</table>
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</section>
  
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<h4> Uploading pictures and files </h4>
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<hr>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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<!-- LOGO BANNER -->
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<ul class="icons" style="text-align:center">
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<li><img src="https://static.igem.org/mediawiki/2015/c/cb/Team_Utah_State_IDTLogopic.png" alt="IDT" height="41" width="150" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/7/7e/Team_Utah_State_CellularEngineeringpic.png" alt="Cellular Engineering" height="46" width="150" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/8/8e/Team_Utah_State_SponsorLogo_Monsato.png" alt="Monsanto" height="51" width="150" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/3/30/Team_Utah_State_SponsorLogo_IMFlash.png" alt="IMFlash" height="43" width="150" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/1/19/Team_Utah_State_SponsorLogo_Schreiber.png" alt="Schriber Foods" height="52" width="150" class="icon"></li>
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<ul class="icons" style="text-align:center">
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<li><img src="https://static.igem.org/mediawiki/2015/7/7a/Team_Utah_State_SponsorLogo_Conagra.png" alt="ConAgra Foods" height="70" width="113" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/1/16/Team_Utah_State_USUBELogo.png" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/0/08/Team_Utah_State_USUCollegeELogo.png" height="82" width="250" class="icon"></li>
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<li><img src="https://static.igem.org/mediawiki/2015/1/1e/Team_Utah_State_SponsorLogo_USUSA.png" alt="USUSA" height="140" widht="150" class="icon"></li>
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</ul> 
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<div class="copyright">
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<ul class="menu" Style="text-align:center;font-size:70%">
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<li>&copy; Untitled. All rights reserved</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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<script src="https://2015.igem.org/Template:Utah_State/jquerymin?action=raw&ctype=text/javascript"></script>
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<script src="https://2015.igem.org/Template:Utah_State/jquerydropotronmin?action=raw&ctype=text/javascript"></script>
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<script src="https://2015.igem.org/Template:Utah_State/skelmin?action=raw&ctype=text/javascript"></script>
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Latest revision as of 22:52, 18 September 2015

Arcana by HTML5 UP

Abstract

The cheese industry generates billions of dollars each year, and no wonder—cheese is delicious! One of the greatest difficulties that the cheese industry faces is bacteriophage (virus) infection, which kills bacteria used in the manufacture of cheese. The industry utilizes a variety of approaches to deal with this problem, each having their own drawbacks. The 2015 USU iGEM team is implementing a synthetic biology approach to design and create phage resistant Lactococcus lactis, a commonly-used cheese starter bacteria. The genetic mechanism behind this phage resistance functions by pairing a promoter that is activated in the presence of phage with a kill switch to terminate infected cells before phage are able to propagate.

Results

Take a look at what we accomplished.


Parts

Here are our Biobricks.

Medal Criteria

Here's what we did to deserve a gold medal.

The Team

Meet the people who made this project possible.

Links to Stuff

Home Team Medal Criteria Attributions
Project Description Experiments and Protocols Results Design
Interlab Study Human Practices Collaborations Safety
All Parts Favorite Parts
  • IDT
  • Cellular Engineering
  • Monsanto
  • IMFlash
  • Schriber Foods
  • ConAgra Foods
  • USUSA
  • © Untitled. All rights reserved
  • Design: HTML5 UP