Difference between revisions of "Team:Pasteur Paris/Week 2"

 
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         <tr  vertical-align:top;>
 
         <tr  vertical-align:top;>
 
             <td class="menunotebook" style="margin: 0px; vertical-align: top;">
 
             <td class="menunotebook" style="margin: 0px; vertical-align: top;">
−
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_1" class="week">
+
                <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_1" class="week">
−
                   <h1>WEEK&nbsp;1</h1>              
+
                   <h1>Week&nbsp;1</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2" class="week">
−
                   <h1>WEEK&nbsp;2</h1>
+
                   <h1>Week&nbsp;2</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_3" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_3" class="week">
−
                   <h1>WEEK&nbsp;3</h1>
+
                   <h1>Week&nbsp;3</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_4" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_4" class="week">
−
                   <h1>WEEK&nbsp;4</h1>
+
                   <h1>Week&nbsp;4</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_5" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_5" class="week">
−
                   <h1>WEEK&nbsp;5</h1>
+
                   <h1>Week&nbsp;5</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_6" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_6" class="week">
−
                   <h1>WEEK&nbsp;6</h1>
+
                   <h1>Week&nbsp;6</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7" class="week">
−
                   <h1>WEEK&nbsp;7</h1>
+
                   <h1>Week&nbsp;7</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8" class="week">
−
                   <h1>WEEK&nbsp;8</h1>
+
                   <h1>Week&nbsp;8</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9" class="week">
−
                   <h1>WEEK&nbsp;9</h1>
+
                   <h1>Week&nbsp;9</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10" class="week">
−
                   <h1>WEEK&nbsp;10</h1>
+
                   <h1>Week&nbsp;10</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_11" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_11" class="week">
−
                   <h1>WEEK&nbsp;11</h1>
+
                   <h1>Week&nbsp;11</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_12" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_12" class="week">
−
                   <h1>WEEK&nbsp;12</h1>
+
                   <h1>Week&nbsp;12</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_13" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_13" class="week">
−
                   <h1>WEEK&nbsp;13</h1>
+
                   <h1>Week&nbsp;13</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_14" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_14" class="week">
−
                   <h1>WEEK&nbsp;14</h1>
+
                   <h1>Week&nbsp;14</h1>
 
               </a>
 
               </a>
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_15" class="week">
 
               <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_15" class="week">
−
                   <h1>WEEK&nbsp;15</h1>
+
                   <h1>Week&nbsp;15</h1>
 
               </a>
 
               </a>
 
             </td>
 
             </td>
−
             <td style="vertical-align: top; width: 80%;" class="fontcorpspage">
+
             <td style="vertical-align: top; width: 90%;" class="fontcorpspage">
−
               <p><span class="titrepage">WEEK&nbsp;2</span><br/><br/>
+
               <p><span class="titrepage">Week&nbsp;2</span><br/><br/>
 
               <span class="soustitrepage">06/08 - 06/12</span></p>
 
               <span class="soustitrepage">06/08 - 06/12</span></p>
−
               <p style="text-align: justify; padding: 30px;">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec et purus vel risus iaculis tristique. Ut sit amet eros mi. Nulla elementum aliquam nibh. Praesent commodo, leo sit amet luctus gravida, dolor orci fermentum dolor, et consequat velit lacus quis ligula. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Fusce ut euismod odio. Vestibulum sed congue velit. Donec vel mi eget felis elementum interdum quis ac nisi.<br/><br/>
+
               <p></p><br>
 +
             
 +
<center><h3><em>Testing of Biobricks by restriction digest</em></h3></center></br>
 +
 
 +
<ol>
 +
  <li style="text-align:left">Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003. </li></br>
 +
  <li style="text-align:left">Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003). </li></br>
  
−
Suspendisse ultricies tortor et elementum aliquam. Sed eu arcu nisi. Sed efficitur semper lacinia. Integer egestas mi id metus aliquam, id porta nisl finibus. Maecenas lobortis felis nec laoreet interdum. Sed tristique accumsan tincidunt. Suspendisse sit amet lacus id massa ultrices luctus quis a urna. Duis euismod turpis vitae urna dictum dictum. Sed ut lacinia augue, interdum euismod risus. Vivamus finibus laoreet quam, quis fringilla elit porta vel. Sed ullamcorper sagittis ante at commodo.<br/><br/>
+
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
 +
<p align="center"><strong><u>Figure 1</u></strong><u>:</u> Verification of  the plasmid purification.
 +
</p>
 +
<br>
 +
<table border="1" cellspacing="0" cellpadding="0" width="768" align="center">
 +
  <tr>
 +
    <td width="153"><p>&nbsp;</p></td>
 +
    <td width="66"><p align="center"><strong>316003</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808007</strong></p></td>
 +
    <td width="85"><p align="center"><strong>808010</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808011</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808012</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808013</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808014</strong></p></td>
 +
    <td width="66"><p align="center"><strong>936000</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="153"><p align="center"><strong>Theoretical size of insert (bp)<strong></p></td>
 +
    <td width="66"><p align="center">1061</p></td>
 +
    <td width="66"><p align="center">4368</p></td>
 +
    <td width="85"><p align="center">948</p></td>
 +
    <td width="66"><p align="center">1011</p></td>
 +
    <td width="66"><p align="center">1242</p></td>
 +
    <td width="66"><p align="center">465</p></td>
 +
    <td width="66"><p align="center">1506</p></td>
 +
    <td width="66"><p align="center">885</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="153"><p align="center"><strong>Inserts present match theoretical values<strong></p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="85"><p align="center">C1/C2 yes<br />
 +
      C3 no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
  </tr>
 +
</table>
  
−
Quisque et consectetur nisi. In eget iaculis nisi. Duis lacinia, ex eu mollis ornare, velit nisl vestibulum mi, at malesuada velit mi in lorem. Suspendisse sollicitudin sapien sit amet lacus pellentesque, non ultrices quam consectetur. Morbi sapien quam, faucibus vitae rhoncus et, volutpat sed metus. Integer odio neque, imperdiet eu luctus at, molestie eget ligula. Morbi vel dui quis erat venenatis iaculis. Vestibulum pulvinar, quam ac tempor efficitur, odio odio congue lectus, quis euismod orci mi a massa.<br/><br/>
+
<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the migration gel (cf. figure 1).<u> </u></p>
 +
<br>
 +
  <li style="text-align:left">Storage at -20°C of BBa_K808007,  BBa_K808014, BBa_K808011 (only for C2/C3). </li>
  
−
Quisque scelerisque sollicitudin convallis. In non semper dui. Proin eu dignissim justo. Suspendisse dictum imperdiet ante quis rhoncus. Nunc mollis tempor orci eget elementum. Morbi sit amet massa eleifend, egestas lectus nec, condimentum ligula. Pellentesque sit amet scelerisque ipsum.<br/><br/>
+
<p>&nbsp;</p>
  
−
Quisque posuere ex sed tortor pulvinar finibus dignissim ac lorem. Vivamus quis ullamcorper odio. Mauris ut semper mi. Phasellus metus nisi, tempor id dolor ac, condimentum scelerisque odio. Vivamus ultricies pellentesque metus. Etiam nec metus felis. Nullam facilisis sit amet ante gravida laoreet. Etiam vulputate commodo semper. Curabitur a rutrum est, id bibendum sem. Sed congue semper mauris, sed laoreet erat pulvinar non.<br/><br/>
+
  <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
−
<a class="hautdepage" href="#"><p><span style="font-size: 60px; font-family: Courier;">^</span><br/><span style="font-size: 20px;">Page up</span></p></a></p>
+
<table border="1" cellspacing="0" cellpadding="0" width="764" align="center">
 +
</br>
 +
  <tr>
 +
    <td width="71" rowspan="2" valign="center"><p><strong>BB</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 1</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 2</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 3</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808007</strong></p></td>
 +
    <td width="72" valign="top"><p>22.60</p></td>
 +
    <td width="79" valign="top"><p>1.99</p></td>
 +
    <td width="79" valign="top"><p>2.04</p></td>
 +
    <td width="72" valign="top"><p>46.90</p></td>
 +
    <td width="79" valign="top"><p>1.92</p></td>
 +
    <td width="79" valign="top"><p>2.09</p></td>
 +
    <td width="72" valign="top"><p>32.30</p></td>
 +
    <td width="79" valign="top"><p>1.90</p></td>
 +
    <td width="79" valign="top"><p>1.79</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808010</strong></p></td>
 +
    <td width="72" valign="top"><p>13.50</p></td>
 +
    <td width="79" valign="top"><p>1.85</p></td>
 +
    <td width="79" valign="top"><p>1.39</p></td>
 +
    <td width="72" valign="top"><p>57.10</p></td>
 +
    <td width="79" valign="top"><p>1.95</p></td>
 +
    <td width="79" valign="top"><p>2.17</p></td>
 +
    <td width="72" valign="top"><p>31.40</p></td>
 +
    <td width="79" valign="top"><p>1.86</p></td>
 +
    <td width="79" valign="top"><p>1.84</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808011</strong></p></td>
 +
    <td width="72" valign="top"><p>37.40</p></td>
 +
    <td width="79" valign="top"><p>1.97</p></td>
 +
    <td width="79" valign="top"><p>2.00</p></td>
 +
    <td width="72" valign="top"><p>61.20</p></td>
 +
    <td width="79" valign="top"><p>1.62</p></td>
 +
    <td width="79" valign="top"><p>1.16</p></td>
 +
    <td width="72" valign="top"><p>21.00</p></td>
 +
    <td width="79" valign="top"><p>1.93</p></td>
 +
    <td width="79" valign="top"><p>1.72</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808012</strong></p></td>
 +
    <td width="72" valign="top"><p>30.20</p></td>
 +
    <td width="79" valign="top"><p>1.93</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="72" valign="top"><p>54.90</p></td>
 +
    <td width="79" valign="top"><p>1.91</p></td>
 +
    <td width="79" valign="top"><p>2.12</p></td>
 +
    <td width="72" valign="top"><p>21.10</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="79" valign="top"><p>1.81</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808013</strong></p></td>
 +
    <td width="72" valign="top"><p>11.50</p></td>
 +
    <td width="79" valign="top"><p>1.91</p></td>
 +
    <td width="79" valign="top"><p>1.11</p></td>
 +
    <td width="72" valign="top"><p>9.60</p></td>
 +
    <td width="79" valign="top"><p>1.78</p></td>
 +
    <td width="79" valign="top"><p>1.42</p></td>
 +
    <td width="72" valign="top"><p>13.90</p></td>
 +
    <td width="79" valign="top"><p>1.74</p></td>
 +
    <td width="79" valign="top"><p>1.21</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808014</strong></p></td>
 +
    <td width="72" valign="top"><p>33.90</p></td>
 +
    <td width="79" valign="top"><p>1.89</p></td>
 +
    <td width="79" valign="top"><p>1.62</p></td>
 +
    <td width="72" valign="top"><p>24.30</p></td>
 +
    <td width="79" valign="top"><p>1.96</p></td>
 +
    <td width="79" valign="top"><p>2.14</p></td>
 +
    <td width="72" valign="top"><p>14.40</p></td>
 +
    <td width="79" valign="top"><p>1.81</p></td>
 +
    <td width="79" valign="top"><p>1.38</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>316003</strong></p></td>
 +
    <td width="72" valign="top"><p>13.60</p></td>
 +
    <td width="79" valign="top"><p>2.04</p></td>
 +
    <td width="79" valign="top"><p>2.38</p></td>
 +
    <td width="72" valign="top"><p>16.50</p></td>
 +
    <td width="79" valign="top"><p>2.10</p></td>
 +
    <td width="79" valign="top"><p>2.41</p></td>
 +
    <td width="72" valign="top"><p>18.80</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="79" valign="top"><p>0.74</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>936000</strong></p></td>
 +
    <td width="72" valign="top"><p>18.20</p></td>
 +
    <td width="79" valign="top"><p>1.98</p></td>
 +
    <td width="79" valign="top"><p>1.19</p></td>
 +
    <td width="72" valign="top"><p>13.10</p></td>
 +
    <td width="79" valign="top"><p>1.97</p></td>
 +
    <td width="79" valign="top"><p>0.93</p></td>
 +
    <td width="72" valign="top"><p>11.90</p></td>
 +
    <td width="79" valign="top"><p>2.16</p></td>
 +
    <td width="79" valign="top"><p>1.18</p></td>
 +
  </tr>
 +
</table>
 +
<p><strong><u>Figure 3</u></strong><u>:</u> Plasmid  purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K316003, BBa_K936000. </p>
 +
<br>
 +
  <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
 +
</ol>
 +
<p>&nbsp;</p>
 +
<p align="center"><img src="https://static.igem.org/mediawiki/2015/9/98/IGEM_Pasteur_gel_2_week_2.png" alt="h" width="448" height="445" /></p>
 +
<p align="center"><strong><u>Figure 4</u></strong><u>:</u> Migration gel of digestion products.<u> </u></p>
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>

Latest revision as of 00:29, 19 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 2

06/08 - 06/12


Testing of Biobricks by restriction digest


  1. Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

    3. h

    Figure 1: Verification of the plasmid purification.


     

    316003

    808007

    808010

    808011

    808012

    808013

    808014

    936000

    Theoretical size of insert (bp)

    1061

    4368

    948

    1011

    1242

    465

    1506

    885

    Inserts present match theoretical values

    no

    yes

    C1/C2 yes
    C3 no

    yes

    no

    no

    yes

    no

    Figure 2: Table of the migration gel (cf. figure 1).


  3. Storage at -20°C of BBa_K808007, BBa_K808014, BBa_K808011 (only for C2/C3).

    4.  

  4. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

    5. BB

    clone 1

    clone 2

    clone 3

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    808007

    22.60

    1.99

    2.04

    46.90

    1.92

    2.09

    32.30

    1.90

    1.79

    808010

    13.50

    1.85

    1.39

    57.10

    1.95

    2.17

    31.40

    1.86

    1.84

    808011

    37.40

    1.97

    2.00

    61.20

    1.62

    1.16

    21.00

    1.93

    1.72

    808012

    30.20

    1.93

    1.94

    54.90

    1.91

    2.12

    21.10

    1.94

    1.81

    808013

    11.50

    1.91

    1.11

    9.60

    1.78

    1.42

    13.90

    1.74

    1.21

    808014

    33.90

    1.89

    1.62

    24.30

    1.96

    2.14

    14.40

    1.81

    1.38

    316003

    13.60

    2.04

    2.38

    16.50

    2.10

    2.41

    18.80

    1.94

    0.74

    936000

    18.20

    1.98

    1.19

    13.10

    1.97

    0.93

    11.90

    2.16

    1.18

    Figure 3: Plasmid purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K316003, BBa_K936000.


  5. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

 

h

Figure 4: Migration gel of digestion products.