Difference between revisions of "Team:Pasteur Paris/Week 2"

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{{Pasteur_Paris}}
 
{{Pasteur_Paris}}
 
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<div class="container">
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  <center>
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      <table width="100%" cellpadding="0" cellspacing="0">
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        <tr  vertical-align:top;>
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            <td class="menunotebook" style="margin: 0px; vertical-align: top;">
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                <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_1" class="week">
 +
                  <h1>Week&nbsp;1</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2" class="week">
 +
                  <h1>Week&nbsp;2</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_3" class="week">
 +
                  <h1>Week&nbsp;3</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_4" class="week">
 +
                  <h1>Week&nbsp;4</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_5" class="week">
 +
                  <h1>Week&nbsp;5</h1>
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              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_6" class="week">
 +
                  <h1>Week&nbsp;6</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7" class="week">
 +
                  <h1>Week&nbsp;7</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8" class="week">
 +
                  <h1>Week&nbsp;8</h1>
 +
              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9" class="week">
 +
                  <h1>Week&nbsp;9</h1>
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              </a>
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              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10" class="week">
 +
                  <h1>Week&nbsp;10</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_11" class="week">
 +
                  <h1>Week&nbsp;11</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_12" class="week">
 +
                  <h1>Week&nbsp;12</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_13" class="week">
 +
                  <h1>Week&nbsp;13</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_14" class="week">
 +
                  <h1>Week&nbsp;14</h1>
 +
              </a>
 +
              <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_15" class="week">
 +
                  <h1>Week&nbsp;15</h1>
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              </a>
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            </td>
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            <td style="vertical-align: top; width: 90%;" class="fontcorpspage">
 +
              <p><span class="titrepage">Week&nbsp;2</span><br/><br/>
 +
              <span class="soustitrepage">06/08 - 06/12</span></p>
 +
              <p></p><br>
 +
             
 +
<center><h3><em>Testing of Biobricks by restriction digest</em></h3></center></br>
 +
 +
<ol>
 +
  <li style="text-align:left">Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li></br>
 +
  <li style="text-align:left">Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003). </li></br>
 +
 +
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
 +
<p align="center"><strong><u>Figure 1</u></strong><u>:</u> Verification of  the plasmid purification.
 +
</p>
 +
<br>
 +
<table border="1" cellspacing="0" cellpadding="0" width="768" align="center">
 +
  <tr>
 +
    <td width="153"><p>&nbsp;</p></td>
 +
    <td width="66"><p align="center"><strong>316003</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808007</strong></p></td>
 +
    <td width="85"><p align="center"><strong>808010</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808011</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808012</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808013</strong></p></td>
 +
    <td width="66"><p align="center"><strong>808014</strong></p></td>
 +
    <td width="66"><p align="center"><strong>936000</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="153"><p align="center"><strong>Theoretical size of insert (bp)<strong></p></td>
 +
    <td width="66"><p align="center">1061</p></td>
 +
    <td width="66"><p align="center">4368</p></td>
 +
    <td width="85"><p align="center">948</p></td>
 +
    <td width="66"><p align="center">1011</p></td>
 +
    <td width="66"><p align="center">1242</p></td>
 +
    <td width="66"><p align="center">465</p></td>
 +
    <td width="66"><p align="center">1506</p></td>
 +
    <td width="66"><p align="center">885</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="153"><p align="center"><strong>Inserts present match theoretical values<strong></p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="85"><p align="center">C1/C2 yes<br />
 +
      C3 no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
    <td width="66"><p align="center">yes</p></td>
 +
    <td width="66"><p align="center">no</p></td>
 +
  </tr>
 +
</table>
 +
 +
<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the migration gel (cf. figure 1).<u> </u></p>
 +
<br>
 +
  <li style="text-align:left">Storage at -20°C of BBa_K808007,  BBa_K808014, BBa_K808011 (only for C2/C3). </li>
 +
 +
<p>&nbsp;</p>
 +
 +
  <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
 +
<table border="1" cellspacing="0" cellpadding="0" width="764" align="center">
 +
</br>
 +
  <tr>
 +
    <td width="71" rowspan="2" valign="center"><p><strong>BB</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 1</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 2</strong></p></td>
 +
    <td width="231" colspan="3" valign="top"><p align="center"><strong>clone 3</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
    <td width="72" valign="top"><p><strong>C (ng/µl)</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/280</strong></p></td>
 +
    <td width="79" valign="top"><p><strong>260/230</strong></p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808007</strong></p></td>
 +
    <td width="72" valign="top"><p>22.60</p></td>
 +
    <td width="79" valign="top"><p>1.99</p></td>
 +
    <td width="79" valign="top"><p>2.04</p></td>
 +
    <td width="72" valign="top"><p>46.90</p></td>
 +
    <td width="79" valign="top"><p>1.92</p></td>
 +
    <td width="79" valign="top"><p>2.09</p></td>
 +
    <td width="72" valign="top"><p>32.30</p></td>
 +
    <td width="79" valign="top"><p>1.90</p></td>
 +
    <td width="79" valign="top"><p>1.79</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808010</strong></p></td>
 +
    <td width="72" valign="top"><p>13.50</p></td>
 +
    <td width="79" valign="top"><p>1.85</p></td>
 +
    <td width="79" valign="top"><p>1.39</p></td>
 +
    <td width="72" valign="top"><p>57.10</p></td>
 +
    <td width="79" valign="top"><p>1.95</p></td>
 +
    <td width="79" valign="top"><p>2.17</p></td>
 +
    <td width="72" valign="top"><p>31.40</p></td>
 +
    <td width="79" valign="top"><p>1.86</p></td>
 +
    <td width="79" valign="top"><p>1.84</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808011</strong></p></td>
 +
    <td width="72" valign="top"><p>37.40</p></td>
 +
    <td width="79" valign="top"><p>1.97</p></td>
 +
    <td width="79" valign="top"><p>2.00</p></td>
 +
    <td width="72" valign="top"><p>61.20</p></td>
 +
    <td width="79" valign="top"><p>1.62</p></td>
 +
    <td width="79" valign="top"><p>1.16</p></td>
 +
    <td width="72" valign="top"><p>21.00</p></td>
 +
    <td width="79" valign="top"><p>1.93</p></td>
 +
    <td width="79" valign="top"><p>1.72</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808012</strong></p></td>
 +
    <td width="72" valign="top"><p>30.20</p></td>
 +
    <td width="79" valign="top"><p>1.93</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="72" valign="top"><p>54.90</p></td>
 +
    <td width="79" valign="top"><p>1.91</p></td>
 +
    <td width="79" valign="top"><p>2.12</p></td>
 +
    <td width="72" valign="top"><p>21.10</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="79" valign="top"><p>1.81</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808013</strong></p></td>
 +
    <td width="72" valign="top"><p>11.50</p></td>
 +
    <td width="79" valign="top"><p>1.91</p></td>
 +
    <td width="79" valign="top"><p>1.11</p></td>
 +
    <td width="72" valign="top"><p>9.60</p></td>
 +
    <td width="79" valign="top"><p>1.78</p></td>
 +
    <td width="79" valign="top"><p>1.42</p></td>
 +
    <td width="72" valign="top"><p>13.90</p></td>
 +
    <td width="79" valign="top"><p>1.74</p></td>
 +
    <td width="79" valign="top"><p>1.21</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>808014</strong></p></td>
 +
    <td width="72" valign="top"><p>33.90</p></td>
 +
    <td width="79" valign="top"><p>1.89</p></td>
 +
    <td width="79" valign="top"><p>1.62</p></td>
 +
    <td width="72" valign="top"><p>24.30</p></td>
 +
    <td width="79" valign="top"><p>1.96</p></td>
 +
    <td width="79" valign="top"><p>2.14</p></td>
 +
    <td width="72" valign="top"><p>14.40</p></td>
 +
    <td width="79" valign="top"><p>1.81</p></td>
 +
    <td width="79" valign="top"><p>1.38</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>316003</strong></p></td>
 +
    <td width="72" valign="top"><p>13.60</p></td>
 +
    <td width="79" valign="top"><p>2.04</p></td>
 +
    <td width="79" valign="top"><p>2.38</p></td>
 +
    <td width="72" valign="top"><p>16.50</p></td>
 +
    <td width="79" valign="top"><p>2.10</p></td>
 +
    <td width="79" valign="top"><p>2.41</p></td>
 +
    <td width="72" valign="top"><p>18.80</p></td>
 +
    <td width="79" valign="top"><p>1.94</p></td>
 +
    <td width="79" valign="top"><p>0.74</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td width="71" valign="top"><p><strong>936000</strong></p></td>
 +
    <td width="72" valign="top"><p>18.20</p></td>
 +
    <td width="79" valign="top"><p>1.98</p></td>
 +
    <td width="79" valign="top"><p>1.19</p></td>
 +
    <td width="72" valign="top"><p>13.10</p></td>
 +
    <td width="79" valign="top"><p>1.97</p></td>
 +
    <td width="79" valign="top"><p>0.93</p></td>
 +
    <td width="72" valign="top"><p>11.90</p></td>
 +
    <td width="79" valign="top"><p>2.16</p></td>
 +
    <td width="79" valign="top"><p>1.18</p></td>
 +
  </tr>
 +
</table>
 +
<p><strong><u>Figure 3</u></strong><u>:</u> Plasmid  purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K316003, BBa_K936000. </p>
 
<br>
 
<br>
−
<p> Week 2 </p>
+
  <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
 +
</ol>
 +
<p>&nbsp;</p>
 +
<p align="center"><img src="https://static.igem.org/mediawiki/2015/9/98/IGEM_Pasteur_gel_2_week_2.png" alt="h" width="448" height="445" /></p>
 +
<p align="center"><strong><u>Figure 4</u></strong><u>:</u> Migration gel of digestion products.<u> </u></p>
 +
            </td>
 +
        </tr>
 +
      </table>
 +
  </center>
 +
</div>
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<style type="text/css">
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Latest revision as of 00:29, 19 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 2

06/08 - 06/12


Testing of Biobricks by restriction digest


  1. Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

    3. h

    Figure 1: Verification of the plasmid purification.


     

    316003

    808007

    808010

    808011

    808012

    808013

    808014

    936000

    Theoretical size of insert (bp)

    1061

    4368

    948

    1011

    1242

    465

    1506

    885

    Inserts present match theoretical values

    no

    yes

    C1/C2 yes
    C3 no

    yes

    no

    no

    yes

    no

    Figure 2: Table of the migration gel (cf. figure 1).


  3. Storage at -20°C of BBa_K808007, BBa_K808014, BBa_K808011 (only for C2/C3).

    4.  

  4. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

    5. BB

    clone 1

    clone 2

    clone 3

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    808007

    22.60

    1.99

    2.04

    46.90

    1.92

    2.09

    32.30

    1.90

    1.79

    808010

    13.50

    1.85

    1.39

    57.10

    1.95

    2.17

    31.40

    1.86

    1.84

    808011

    37.40

    1.97

    2.00

    61.20

    1.62

    1.16

    21.00

    1.93

    1.72

    808012

    30.20

    1.93

    1.94

    54.90

    1.91

    2.12

    21.10

    1.94

    1.81

    808013

    11.50

    1.91

    1.11

    9.60

    1.78

    1.42

    13.90

    1.74

    1.21

    808014

    33.90

    1.89

    1.62

    24.30

    1.96

    2.14

    14.40

    1.81

    1.38

    316003

    13.60

    2.04

    2.38

    16.50

    2.10

    2.41

    18.80

    1.94

    0.74

    936000

    18.20

    1.98

    1.19

    13.10

    1.97

    0.93

    11.90

    2.16

    1.18

    Figure 3: Plasmid purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K316003, BBa_K936000.


  5. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

 

h

Figure 4: Migration gel of digestion products.