Difference between revisions of "Team:Pasteur Paris/Week 2"

 
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               <p><span class="titrepage">Week&nbsp;2</span><br/><br/>
 
               <p><span class="titrepage">Week&nbsp;2</span><br/><br/>
 
               <span class="soustitrepage">06/08 - 06/12</span></p>
 
               <span class="soustitrepage">06/08 - 06/12</span></p>
−
               <p></p><br><br><br>
+
               <p></p><br>
 
                
 
                
 +
<center><h3><em>Testing of Biobricks by restriction digest</em></h3></center></br>
 +
 
<ol>
 
<ol>
−
   <li style="text-align:left">Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li>
+
   <li style="text-align:left">Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li></br>
−
   <li style="text-align:left">Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003). </li>
+
   <li style="text-align:left">Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003). </li></br>
  
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
Line 117: Line 119:
 
     <td width="66"><p align="center"><strong>808007</strong></p></td>
 
     <td width="66"><p align="center"><strong>808007</strong></p></td>
 
     <td width="85"><p align="center"><strong>808010</strong></p></td>
 
     <td width="85"><p align="center"><strong>808010</strong></p></td>
−
     <td width="132"><p align="center"><strong>808011</strong></p></td>
+
     <td width="66"><p align="center"><strong>808011</strong></p></td>
 
     <td width="66"><p align="center"><strong>808012</strong></p></td>
 
     <td width="66"><p align="center"><strong>808012</strong></p></td>
 
     <td width="66"><p align="center"><strong>808013</strong></p></td>
 
     <td width="66"><p align="center"><strong>808013</strong></p></td>
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     <td width="66"><p align="center">4368</p></td>
 
     <td width="66"><p align="center">4368</p></td>
 
     <td width="85"><p align="center">948</p></td>
 
     <td width="85"><p align="center">948</p></td>
−
     <td width="132"><p align="center">1011</p></td>
+
     <td width="66"><p align="center">1011</p></td>
 
     <td width="66"><p align="center">1242</p></td>
 
     <td width="66"><p align="center">1242</p></td>
 
     <td width="66"><p align="center">465</p></td>
 
     <td width="66"><p align="center">465</p></td>
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     <td width="85"><p align="center">C1/C2 yes<br />
 
     <td width="85"><p align="center">C1/C2 yes<br />
 
       C3 no</p></td>
 
       C3 no</p></td>
−
     <td width="132"><p align="center">yes</p></td>
+
     <td width="66"><p align="center">yes</p></td>
 
     <td width="66"><p align="center">no</p></td>
 
     <td width="66"><p align="center">no</p></td>
 
     <td width="66"><p align="center">no</p></td>
 
     <td width="66"><p align="center">no</p></td>
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<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the migration gel (cf. figure 1).<u> </u></p>
 
<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the migration gel (cf. figure 1).<u> </u></p>
 
<br>
 
<br>
−
   <li style="text-align:left">Storage at -20°C of BBa_808007,  BBa_808014, BBa_808011 (only for C2/C3). </li>
+
   <li style="text-align:left">Storage at -20°C of BBa_K808007,  BBa_K808014, BBa_K808011 (only for C2/C3). </li>
  
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
  
−
   <li style="text-align:left">Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
+
   <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
 
<table border="1" cellspacing="0" cellpadding="0" width="764" align="center">
 
<table border="1" cellspacing="0" cellpadding="0" width="764" align="center">
 +
</br>
 
   <tr>
 
   <tr>
 
     <td width="71" rowspan="2" valign="center"><p><strong>BB</strong></p></td>
 
     <td width="71" rowspan="2" valign="center"><p><strong>BB</strong></p></td>
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   </tr>
 
   </tr>
 
</table>
 
</table>
−
<p><strong><u>Figure 3</u></strong><u>:</u> Plasmid  purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013,  BBa_808014, BBa_316003, BBa_936000. </p>
+
<p><strong><u>Figure 3</u></strong><u>:</u> Plasmid  purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K316003, BBa_K936000. </p>
 
<br>
 
<br>
−
   <li style="text-align:left">Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
+
   <li style="text-align:left">Plasmid  purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000. </li>
 
</ol>
 
</ol>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>

Latest revision as of 00:29, 19 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 2

06/08 - 06/12


Testing of Biobricks by restriction digest


  1. Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

    3. h

    Figure 1: Verification of the plasmid purification.


     

    316003

    808007

    808010

    808011

    808012

    808013

    808014

    936000

    Theoretical size of insert (bp)

    1061

    4368

    948

    1011

    1242

    465

    1506

    885

    Inserts present match theoretical values

    no

    yes

    C1/C2 yes
    C3 no

    yes

    no

    no

    yes

    no

    Figure 2: Table of the migration gel (cf. figure 1).


  3. Storage at -20°C of BBa_K808007, BBa_K808014, BBa_K808011 (only for C2/C3).

    4.  

  4. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

    5. BB

    clone 1

    clone 2

    clone 3

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    C (ng/µl)

    260/280

    260/230

    808007

    22.60

    1.99

    2.04

    46.90

    1.92

    2.09

    32.30

    1.90

    1.79

    808010

    13.50

    1.85

    1.39

    57.10

    1.95

    2.17

    31.40

    1.86

    1.84

    808011

    37.40

    1.97

    2.00

    61.20

    1.62

    1.16

    21.00

    1.93

    1.72

    808012

    30.20

    1.93

    1.94

    54.90

    1.91

    2.12

    21.10

    1.94

    1.81

    808013

    11.50

    1.91

    1.11

    9.60

    1.78

    1.42

    13.90

    1.74

    1.21

    808014

    33.90

    1.89

    1.62

    24.30

    1.96

    2.14

    14.40

    1.81

    1.38

    316003

    13.60

    2.04

    2.38

    16.50

    2.10

    2.41

    18.80

    1.94

    0.74

    936000

    18.20

    1.98

    1.19

    13.10

    1.97

    0.93

    11.90

    2.16

    1.18

    Figure 3: Plasmid purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K316003, BBa_K936000.


  5. Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.

 

h

Figure 4: Migration gel of digestion products.