Difference between revisions of "Team:Duke/Experiments"
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<h4 class="subhead">Experiments</h4> | <h4 class="subhead">Experiments</h4> | ||
<h4 class="subhead">Protocols</h4> | <h4 class="subhead">Protocols</h4> | ||
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| + | <p> BsaI is a restriction enzyme with unique type 2 properties. What this means is that a sequence of DNA can be designed to have a desired overhang on each side. The BsaI will cut itself out of the sequence, as shown in the picture below: </p> <br> | ||
<br><img src="https://static.igem.org/mediawiki/2015/thumb/e/e6/2015Duke-gRNA1.jpeg/800px-2015Duke-gRNA1.jpeg" width="550";><br> | <br><img src="https://static.igem.org/mediawiki/2015/thumb/e/e6/2015Duke-gRNA1.jpeg/800px-2015Duke-gRNA1.jpeg" width="550";><br> | ||
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| + | <p> After cutting its recognition sequence out and leaving the desired overhang, a gRNA with matching sticky ends can be inserted into the space. In other words, the gRNA will completely take the place of the BsaI restriction site. </p> <br> | ||
<br><img src="https://static.igem.org/mediawiki/2015/thumb/8/8c/2015Duke-gRNA2.jpeg/800px-2015Duke-gRNA2.jpeg" width="550";><br> | <br><img src="https://static.igem.org/mediawiki/2015/thumb/8/8c/2015Duke-gRNA2.jpeg/800px-2015Duke-gRNA2.jpeg" width="550";><br> | ||
Revision as of 02:05, 19 September 2015
Experiments & Protocols
Experiments
Protocols
BsaI is a restriction enzyme with unique type 2 properties. What this means is that a sequence of DNA can be designed to have a desired overhang on each side. The BsaI will cut itself out of the sequence, as shown in the picture below:
After cutting its recognition sequence out and leaving the desired overhang, a gRNA with matching sticky ends can be inserted into the space. In other words, the gRNA will completely take the place of the BsaI restriction site.
