Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/1 July 2015"
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Plates were streaked two weeks ago with transformed E-coli cells containing an alternating sequence of wild type silk and GFP, specifically: N-terminus-GFP-C-terminus. An isolated colony was picked and inoculated in Lysogeny Broth (commonly referred to as Luria broth, an excellent growth medium for bacteria) overnight for 16 hours. | Plates were streaked two weeks ago with transformed E-coli cells containing an alternating sequence of wild type silk and GFP, specifically: N-terminus-GFP-C-terminus. An isolated colony was picked and inoculated in Lysogeny Broth (commonly referred to as Luria broth, an excellent growth medium for bacteria) overnight for 16 hours. | ||
| − | Procedure | + | <u>Procedure</u> |
| − | + | <p>Colony picked with micropipet and inserted into a culture tube containing 10 mL of LB and 10 ul of Ampicillin. | |
| − | Colony picked with micropipet and inserted into a culture tube containing 10 mL of LB and 10 ul of Ampicillin. | + | |
Latest revision as of 23:07, 9 July 2015
Inoculation of Tamura Colony
Plates were streaked two weeks ago with transformed E-coli cells containing an alternating sequence of wild type silk and GFP, specifically: N-terminus-GFP-C-terminus. An isolated colony was picked and inoculated in Lysogeny Broth (commonly referred to as Luria broth, an excellent growth medium for bacteria) overnight for 16 hours.
Procedure
Colony picked with micropipet and inserted into a culture tube containing 10 mL of LB and 10 ul of Ampicillin.