Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/10 May 2015"

(Created page with "='''Transformation with pET24a'''= 1. Thaw 50uL of chemically competent cells for 10 minutes on ice. 2. Add 1uL of pET expression vector to cells, incubate for 5 minutes. 3. Res...")
 
 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics"><li>Spider Silk Genetics</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk"><li>Honeybee Silk</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Recombinant_Expression"><li>Recombinant Silk Functionalization</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Materials"><li>Materials  Processing</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Attributions"><li>ATTRIBUTIONS</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Collaborations"><li>COLLABORATIONS</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Practices"><li>HUMAN PRACTICES</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Safety"><li>SAFETY</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Modeling"><li>MODELING</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Measurement"><li>MEASUREMENT STUDY</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Software"><li>SOFTWARE</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Entrepreneurship"><li>ENTREPRENEURSHIP</li></a>
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='''Transformation with pET24a'''=
 
='''Transformation with pET24a'''=
  
−
1. Thaw 50uL of chemically competent cells for 10 minutes on ice.
+
#Thaw 50uL of chemically competent cells for 10 minutes on ice.
−
2. Add 1uL of pET expression vector to cells, incubate for 5 minutes.
+
#Add 1uL of pET expression vector to cells, incubate for 5 minutes.
−
3. Rescue in 200uL of SOC, incubate at 37C for 1 hour.
+
# Rescue in 200uL of SOC, incubate at 37C for 1 hour.
−
4. Plate 100uL on kanamycin plates, spread with beads.
+
# Plate 100uL on kanamycin plates, spread with beads.
−
5. Incubate at 37C.
+
# Incubate at 37C.

Latest revision as of 06:27, 10 July 2015


Transformation with pET24a

  1. Thaw 50uL of chemically competent cells for 10 minutes on ice.
  2. Add 1uL of pET expression vector to cells, incubate for 5 minutes.
  3. Rescue in 200uL of SOC, incubate at 37C for 1 hour.
  4. Plate 100uL on kanamycin plates, spread with beads.
  5. Incubate at 37C.