Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/21 May 2015"

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<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
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<ul>
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<a href="https://2015.igem.org/Team:UCLA"><li>HOME</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Team"><li>TEAM</li></a>
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<a href="#"><li>PROJECTS
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            <ul>
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<!-- <a href="https://2015.igem.org/Team:UCLA/Description"><li>Description</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Experiments"><li>Experiments &amp; Protocols</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Results"><li>Results</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Design"><li>Design</li></a>-->
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<a href="https://2015.igem.org/Team:UCLA/Projects/Silks"><li>Silk Genetics</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Projects/Functionalization"><li>Functionalization</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Projects/Materials"><li>Materials Processing</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Projects/Protein_Cages"><li>Protein Cages</li></a>
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</ul>
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</li></a>
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<a href="#"><li>PARTS
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            <ul>
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<a href="https://2015.igem.org/Team:UCLA/Parts"><li>Team Parts</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Basic_Part"><li>Basic Parts</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Composite_Part"><li>Composite Parts</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Part_Collection"><li>Part Collection</li></a> 
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Notebook"><li>NOTEBOOKS
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                                                <ul>
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics"><li>Spider Silk Genetics</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk"><li>Honeybee Silk</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Recombinant_Expression"><li>Recombinant Silk Functionalization</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Materials"><li>Materials  Processing</li></a> 
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Protein_Cages"><li>Protein Cages</li></a>
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                                                        <a href="https://2015.igem.org/Team:UCLA/Notebook/Interlab_Study"><li>UCLA Measurement Interlab Study</li></a>
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</ul>
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                                        </li></a>
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<a href="#"><li>MEETING NOTES
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                                                <ul>
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<a href="https://2015.igem.org/Team:UCLA/Notebook/General_Meetings"><li>General Meetings</li></a>
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                                                        <a href="https://2015.igem.org/Team:UCLA/Notebook/Coordinator_Meetings"><li>Coordinator Meetings</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Notebook/Advisor_Meetings"><li>Advisor Meetings</li></a>   
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</ul>
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                                        </li></a>
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 +
     
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<a href="https://2015.igem.org/Team:UCLA/Attributions"><li>ATTRIBUTIONS</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Collaborations"><li>COLLABORATIONS</li></a>
 +
 +
<a href="https://2015.igem.org/Team:UCLA/Practices"><li>HUMAN PRACTICES</li></a>
 +
 +
<a href="https://2015.igem.org/Team:UCLA/Safety"><li>SAFETY</li></a>
 +
 +
<a href="https://2015.igem.org/Team:UCLA/Modeling"><li>MODELING</li></a>
 +
 +
<a href="https://2015.igem.org/Team:UCLA/Measurement"><li>MEASUREMENT STUDY</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Software"><li>SOFTWARE</li></a>
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<a href="https://2015.igem.org/Team:UCLA/Entrepreneurship"><li>ENTREPRENEURSHIP</li></a>
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</ul>
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</div>
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='''Inoculation of 5/10 Transformation'''=
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*I picked four colonies from the 1:1 kanamycin plate and suspended each in 100uL ddH2O
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*Each sample was inoculated in 3 different culture tubes with 5mL LB broth and 5uL 1000X kanamycin and was incubated at 37C.

Revision as of 21:15, 10 July 2015