Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/14 July 2015"
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Both samples were sent in for sequencing. | Both samples were sent in for sequencing. | ||
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| + | =Calculating Competency= | ||
| + | *Calculating competency of cells prepared on 7/12. | ||
| + | *Equation = # colonies / ug DNA plated | ||
| + | *Using 2 ul of 50pg/ul puc19 vector to transform in to 50 ul | ||
| + | *Counted 300 colonies | ||
| + | *plated 1x10^-5 ug (1/10 of tranformation reacction). | ||
| + | *Therefore tranformation efficiency = '''3 x 10^7''' cfu/ug DNA | ||
Revision as of 21:41, 14 July 2015
Miniprep
Samples 1 and 3 (inoculated) were miniprepped using the Zymo Plasmid Miniprep Kit.
OD
- 128.23 ng/uL
- 106.85 ng/uL
Both samples were sent in for sequencing.
Calculating Competency
- Calculating competency of cells prepared on 7/12.
- Equation = # colonies / ug DNA plated
- Using 2 ul of 50pg/ul puc19 vector to transform in to 50 ul
- Counted 300 colonies
- plated 1x10^-5 ug (1/10 of tranformation reacction).
- Therefore tranformation efficiency = 3 x 10^7 cfu/ug DNA