Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/4 August 2015"
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| + | '''<u>BCA Assay for Concentrated Fraction</u>''' | ||
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'''<u>Start-Up Culture for 12-mer Expression</u> | '''<u>Start-Up Culture for 12-mer Expression</u> | ||
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| + | The Silk Genetics Team streaked a plate with their sequence-verified 12-mer proteins. A colony was picked and pipetted into 10mL of autoclaved LB and 10ul of Chloramphenicol. Two start-up cultures were prepared to ensure at least one is in the optimal OD range for 1 L culturing. The tubes were put into a shaking incubator at 37 degrees Celsius and left overnight for 17 hours. | ||
Revision as of 08:48, 6 August 2015
BCA Assay for Concentrated Fraction
Start-Up Culture for 12-mer Expression
The Silk Genetics Team streaked a plate with their sequence-verified 12-mer proteins. A colony was picked and pipetted into 10mL of autoclaved LB and 10ul of Chloramphenicol. Two start-up cultures were prepared to ensure at least one is in the optimal OD range for 1 L culturing. The tubes were put into a shaking incubator at 37 degrees Celsius and left overnight for 17 hours.