Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/10 August 2015"

(→‎Redid PCR to amplify M1-12 ICA product)
(→‎Redid PCR to amplify M1-12 ICA product)
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*Changed extension time to 40 sec instead of 35 sec
 
−
* Used new primers from stock
 
  
−
Ran samples on 1% gel with 1kb ladder
 
−
 
−
Gel purified: 11ng/uL in 30uL
 
 
{| class="wikitable"
 
{| class="wikitable"
 
| 98C
 
| 98C
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| Hold
 
| Hold
 
|}
 
|}
 +
 +
*Changed extension time to 40 sec instead of 35 sec
 +
* Used new primers from stock
 +
 +
Ran samples on 1% gel with 1kb ladder
 +
 +
Gel purified: 11ng/uL in 30uL

Revision as of 01:52, 11 August 2015

iGEM UCLA




08/10/2015

Redid PCR to amplify M1-12 ICA product

5X Q5 buff 10 uL
10mM dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
Template 1 uL
ddH2O 32.5 uL
Q5 0.5 uL


98C 30 sec
98C 10 sec
66C 20 sec
72C 40 sec
x23
72C 2 min
12 C Hold
  • Changed extension time to 40 sec instead of 35 sec
  • Used new primers from stock

Ran samples on 1% gel with 1kb ladder

Gel purified: 11ng/uL in 30uL