Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/12 August 2015"
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Revision as of 00:11, 13 August 2015
Multiple Mini Expressions: Optimizing protein yield
- Today we are testing multiple growth conditions of our bl21 cells for total cell and protein yield. Parameters we are optimizing are iptg concentration, and growth temperature.
| IPTG concentration/Temp | 37C | 30C |
|---|---|---|
| 0.1 mM | ||
| 0.5 mM | ||
| 1.0 mM | ||
| 2.0 mM |