Difference between revisions of "Team:Pasteur Paris/Week 1"
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<p style="text-align: justify; padding: 30px;"> | <p style="text-align: justify; padding: 30px;"> | ||
− | <center> | + | <center> 06-04-15 : Transformation of our biobricks in DH5-alpha competent cells : xxxxxxxx </center> |
<br> | <br> | ||
<ul> Materials : | <ul> Materials : | ||
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<br> | <br> | ||
<br> | <br> | ||
− | |||
− | + | <center>06-05-15 : Bacterial amplification : xxxxxxxxxxx</center> | |
− | + | ||
− | + | ||
− | + | ||
− | + | <ul>Materials : | |
− | + | <li>Petri dishes</li> | |
− | + | <li>LB with or without chlroramphénicol</li> | |
− | + | <li>15mL falcon tubes (3 per Petridish used)</li> | |
+ | </ul> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | <ol>Protocol : | ||
+ | <li>Fill the tubes with 5mL of the correct media (3 tubes per strain)</li> | ||
+ | <li>Pick three transformed clones per Petri box (1 clone per 15mL tube)</li> | ||
+ | <li>Incubate in agitation (8 hours at 200 rpm, 37°C)</li> | ||
+ | </ol> | ||
Revision as of 12:33, 18 August 2015
WEEK 106/01 - 06/05 WEEK 206/08 - 06/12 WEEK 306/15 - 06/19 WEEK 406/22 - 06/26 WEEK 506/29 - 07/03 WEEK 607/06 - 07/10 WEEK 707/13 - 07/17 WEEK 807/20 - 07/24 WEEK 907/27 - 07/31 WEEK 1008/03 - 08/07 WEEK 1108/10 - 08/14 WEEK 1208/17 - 08/21 WEEK 1308/24 - 08/28 WEEK 1408/31 - 09/04 WEEK 1509/07 - 09/11 |
WEEK 1
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