Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/26 August 2015"
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Latest revision as of 18:50, 31 August 2015
8/26/2015
Transformation Results
- Only 2 colonies present on the M1/2[1:2]-12(1C3) plate.
- Possibly due to DNA loss during PCR purification.
Miniprep and Sequencing
- Used Qiagen to miniprep 5 mL culture samples M1-12(1C3), M1/2[1:1]-12(1C3)/(T7), M1/2[2:1]-12(1C3)/(T7).
- Sent to Genewiz for sequencing.
- Used VF and VR primers for each.
Liquid Culture
- Picked the two colonies into 5 mL LB + chlor.