Difference between revisions of "Team:Pasteur Paris/Notebook"

Line 268: Line 268:
 
         <div class="row">           
 
         <div class="row">           
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7">
             <div class="col-lg-12" id="week_7" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/07/Week7.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_7')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12" id="week_7" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/07/Week7.JPG)" onmouseover="Help('We performed our first PCR amplification of the genes that we would use for our Gibson Assembly and we checked if the PCR was done correctly by performing an electrophoresis on agarose.', 'week_7')" onmouseout="EfaceHelp()">
 
               Week 7 <p>06/13 - 07/17</p>
 
               Week 7 <p>06/13 - 07/17</p>
 
             </div>
 
             </div>
Line 281: Line 281:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8">
             <div class="col-lg-12" id="week_8" style=" background-image:url(https://static.igem.org/mediawiki/2015/8/88/Week8.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_8')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12" id="week_8" style=" background-image:url(https://static.igem.org/mediawiki/2015/8/88/Week8.JPG)" onmouseover="Help('We performed stab cultures on the biobricks that we already had, in order to store them.', 'week_8')" onmouseout="EfaceHelp()">
 
               Week 8 <p>07/20 - 07/24</p>
 
               Week 8 <p>07/20 - 07/24</p>
 
             </div>
 
             </div>
Line 294: Line 294:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9">
             <div class="col-lg-12" id="week_9" style=" background-image:url(https://static.igem.org/mediawiki/2015/9/9e/DSCN1936.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_9')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12" id="week_9" style=" background-image:url(https://static.igem.org/mediawiki/2015/9/9e/DSCN1936.JPG)" onmouseover="Help('We did a bacterial culture of DH5-alpha bacteria harboring the 808030 Biobrick.', 'week_9')" onmouseout="EfaceHelp()">
 
               Week 9 <p>07/27 - 07/31</p>
 
               Week 9 <p>07/27 - 07/31</p>
 
             </div>
 
             </div>
Line 307: Line 307:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10">
             <div class="col-lg-12" id="week_10" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/48/Week10.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_10')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12" id="week_10" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/48/Week10.JPG)" onmouseover="Help('The week was spent testing the toxicity, solubility and use of TPA as a carbon source by the bacteria BAP1. In order to do this, we prepared different types of medium. This week, we also purified and ligated BBa_808030 and the plasmid Backbone pDG011 and checking if the ligation had worked.
 +
We also prepared the fragments for the Gibson assembly through PCR Amplification or ligation, depending on the fragment.', 'week_10')" onmouseout="EfaceHelp()">
 
               Week 10 <p>08/03 - 08/07</p>
 
               Week 10 <p>08/03 - 08/07</p>
 
             </div>
 
             </div>

Revision as of 09:11, 14 September 2015

Notebook

Search for a content: