Difference between revisions of "Team:Czech Republic/Protocols"

(Soft lithography - Bonding PDMS-Glass)
(Preparation of the substrates)
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==== Preparation of the substrates ====
 
==== Preparation of the substrates ====
  
Glass slide
+
===== Glass slide =====
  
 
*2.5 minutes air plasma treatment
 
*2.5 minutes air plasma treatment

Revision as of 14:38, 14 September 2015

Protocols

Purification

....

Restriction

...

GA

...

Soft lithography - PDMS molding

  • Silicon masters
  • 40g PDMS + 4g curing agent
  • curing 80°C/2h

Soft lithography - Bonding PDMS-Glass

Preparation of the substrates

Glass slide
  • 2.5 minutes air plasma treatment

PDMS

  • Cut the PDMS

Air plasma treatment

  • Position the substrate

Permanent irreversible bonding

  • Place PDMS over glass slide
  • post-bake 80°C/1h

...