Difference between revisions of "Team:Vilnius-Lithuania/Description"
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− | + | <p style="border-left: 5px solid rgb(236,151,31); padding-left: 3px; border-bottom: none">Diagram 1. The CRISPR-Cas system</p> | |
<p class="text-justify">We use this bacterial protection CRISPR-Cas system to create a method by which we could programme the life of genetically modified bacteria. One of the main goals of our project is regulating a specific number of life cycles that genetically altered bacteria could go through, when it is released into natural wildlife conditions. This is Coliclock – an automatic timer, which starts when you set the bacteria free to do its function.</p> | <p class="text-justify">We use this bacterial protection CRISPR-Cas system to create a method by which we could programme the life of genetically modified bacteria. One of the main goals of our project is regulating a specific number of life cycles that genetically altered bacteria could go through, when it is released into natural wildlife conditions. This is Coliclock – an automatic timer, which starts when you set the bacteria free to do its function.</p> | ||
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− | + | <p style="border-left: 5px solid rgb(236,151,31); padding-left: 3px; border-bottom: none">Diagram 2. The principle</p> | |
<p class="text-justify">This is the point at which our system switches from laboratory mode into wildlife mode. Once the concentration of cI repressor reaches a minor treshold, the transcription of LuxR and LuxI begins. At first it is not very indicative, but through the positive loop expression increases dramatically. </p> | <p class="text-justify">This is the point at which our system switches from laboratory mode into wildlife mode. Once the concentration of cI repressor reaches a minor treshold, the transcription of LuxR and LuxI begins. At first it is not very indicative, but through the positive loop expression increases dramatically. </p> | ||
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<img src="https://static.igem.org/mediawiki/2015/8/83/Vilnius15_loop.png" style="width: 500px; " /> | <img src="https://static.igem.org/mediawiki/2015/8/83/Vilnius15_loop.png" style="width: 500px; " /> | ||
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+ | <p style="border-left: 5px solid rgb(236,151,31); padding-left: 3px; border-bottom: none">Diagram 3. Positive loop</p> | ||
<p class="text-justify">The product of LuxI sequence is HL (homoserine lactone, which, together with the LuxR protein, acts as an activation complex for pLux/cl promoters. This creates a positive loop of expression – the first transcribed proteins join into a complex, which activates the further transcription of the same proteins. The genes within these promoters are intensively transcribed. We call this whole construct the "switch-on" of the functional Coliclock system subunit.</p> | <p class="text-justify">The product of LuxI sequence is HL (homoserine lactone, which, together with the LuxR protein, acts as an activation complex for pLux/cl promoters. This creates a positive loop of expression – the first transcribed proteins join into a complex, which activates the further transcription of the same proteins. The genes within these promoters are intensively transcribed. We call this whole construct the "switch-on" of the functional Coliclock system subunit.</p> |
Revision as of 12:44, 16 September 2015