Week 2

06/08 - 06/12

1. Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).



Figure 1: Verification of the plasmid purification.




	316003	808007	808010	808011	808012	808013	808014	936000
Theoretical size of insert (bp)	1061	4368	948	1011	1242	465	1506	885
Inserts present match theoretical values	no	yes	C1/C2 yes C3 no	yes	no	no	yes	no

Figure 2: Table of the migration gel (cf. figure 1).



3. Storage at -20°C of BBa_808007, BBa_808014, BBa_808011 (only for C2/C3).

 

4. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

BB	clone 1			clone 2			clone 3
	C (ng/µl)	260/280	260/230	C (ng/µl)	260/280	260/230	C (ng/µl)	260/280	260/230
808007	22.60	1.99	2.04	46.90	1.92	2.09	32.30	1.90	1.79
808010	13.50	1.85	1.39	57.10	1.95	2.17	31.40	1.86	1.84
808011	37.40	1.97	2.00	61.20	1.62	1.16	21.00	1.93	1.72
808012	30.20	1.93	1.94	54.90	1.91	2.12	21.10	1.94	1.81
808013	11.50	1.91	1.11	9.60	1.78	1.42	13.90	1.74	1.21
808014	33.90	1.89	1.62	24.30	1.96	2.14	14.40	1.81	1.38
316003	13.60	2.04	2.38	16.50	2.10	2.41	18.80	1.94	0.74
936000	18.20	1.98	1.19	13.10	1.97	0.93	11.90	2.16	1.18

Figure 3: Plasmid purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013, BBa_808014, BBa_316003, BBa_936000.



5. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

Figure 4: Migration gel of digestion products.