Difference between revisions of "Team:Pasteur Paris/Week 4"
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<td width="197" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td> | <td width="197" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td> | ||
− | <td width=" | + | <td width="76" valign="top"><p align="center"><strong>Clone 1 </strong></p></td> |
− | <td width=" | + | <td width="79" valign="top"><p align="center"><strong>Clone 2 </strong></p></td> |
− | <td width=" | + | <td width="80" valign="top"><p align="center"><strong>Clone 3 </strong></p></td> |
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<p align="left">- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" /> | <p align="left">- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" /> | ||
− | <strong><u>Figure 6</u></strong><u>:</u> Results of the | + | <strong><u>Figure 6</u></strong><u>:</u> Results of the gel migration (BBa_808026). Three different colonies (C1-C3 shown) </p><br> |
<li style="text-align:left">Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34. </li><br> | <li style="text-align:left">Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34. </li><br> | ||
<li style="text-align:left">Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li> | <li style="text-align:left">Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li> | ||
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<td width="197" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td> | <td width="197" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td> | ||
− | <td width=" | + | <td width="76" valign="top"><p align="center"><strong>Clone 1 </p></td> |
− | <td width=" | + | <td width="79" valign="top"><p align="center"><strong>Clone 2 </p></td> |
− | <td width=" | + | <td width="80" valign="top"><p align="center"><strong>Clone 3 </p></td> |
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<p align="center"><strong><u>Figure 7:</u></strong> DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. </p><br> | <p align="center"><strong><u>Figure 7:</u></strong> DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. </p><br> | ||
− | <li style="text-align:left">Transformation of plasmids TeV and pDG022 in DH5-⍺ chemically competent cells. </li> | + | <li style="text-align:left">Transformation of plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-⍺ chemically competent cells. </li> |
</ol> | </ol> | ||
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Revision as of 19:53, 17 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 4
Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. - OD measurement using a Spectrophotometer:
- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V): Figure 6: Results of the gel migration (BBa_808026). Three different colonies (C1-C3 shown)
Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest. |