Difference between revisions of "Team:Amsterdam/Project/Phy param/Synechocysytis"

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   <img src="https://static.igem.org/mediawiki/2015/3/3b/Amsterdam_qp_lactate.png" alt="Lactate Qp and growth">
 
   <img src="https://static.igem.org/mediawiki/2015/3/3b/Amsterdam_qp_lactate.png" alt="Lactate Qp and growth">
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   <img src="https://static.igem.org/mediawiki/2015/6/68/Amsterdam_qp_acetate.png" alt="Acetate Qp and growth">
 
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    <th class="tg-031e">Strain<br></th>
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    <th class="tg-031e">growth rate<br></th>
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    <th class="tg-031e"></th>
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  <tr>
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    <td class="tg-031e">SAA023<br></td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  <tr>
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    <td class="tg-031e">Syn1413</td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  </tr>
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  <tr>
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    <td class="tg-031e">Δacs</td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  </tr>
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  <tr>
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    <td class="tg-031e">Pta1</td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  </tr>
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  <tr>
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    <td class="tg-031e">Pta2<br></td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  </tr>
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  <tr>
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    <td class="tg-031e">ack</td>
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    <td class="tg-031e"></td>
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    <td class="tg-031e"></td>
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  </tr>
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</table>
 
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Revision as of 20:49, 17 September 2015

iGEM Amsterdam 2015

Synechocystis physiological parameters

Counting cells, counting molecules

Overview

Background

Estimating physiological parameters is necessary for building meaningful models that can help us to rationally design our consortium. In addition production stability can be assessed by estimating this parameters over a long period of time give.

Aim

We aim to use different cultivation strategies to Characterize growth and production rates of carbon producing Synechocystis strains their stability.

Approach

We have used turbidostat cultivation system to determine the stability of the physiological parameters

Results

  • We have demonstrated the instability of classical engineering strategies. As shown in figure 1 the strain SAA023 lose the ability to produce lactate after 300 hours of continuous culture.
  • We show that the Qp in the growth coupled producer Δacs remains constant over the time (figure 2).
  • We have estimated Qp and growth rates for six Synechocystis.
.

Lactate Qp and growth
Figure 1. - Growth and Qp of strain SAA023 over 900 hours of continuous culture showing the drop in production.
Acetate Qp and growth
Figure 2. - Growth and Qp of strain Δacs over 900 hours of continuous culture showing a constant production over the duration of the experiment.
Strain
 growth rate
SAA023
Syn1413
Δacs
Pta1
Pta2
ack