Difference between revisions of "Team:Pasteur Paris/Week 10"
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<li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li> | <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li> | ||
<li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li> | <li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li> | ||
− | + | <li style="text-align:left">Solubilization of TPA by NaOH</li> | |
− | + | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li> | |
− | Creation of a stock solution of soluble TPA in water at 0,5mol/L | + | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li> |
− | Test of the TPA | + | <li style="text-align:left"><u>Preparation of different media containing TPA and EG:</u></li> |
− | + | ||
− | Preparation of different media containing TPA and EG : | + | |
Media 1 to 10 are made in solid form | Media 1 to 10 are made in solid form | ||
Revision as of 12:19, 18 September 2015
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Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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