Difference between revisions of "Team:Pasteur Paris/Week 10"
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<li style="text-align:left">Growth curve of BAP 1 in media 11 to 14 in order to assess TPA toxicity.</li></br> | <li style="text-align:left">Growth curve of BAP 1 in media 11 to 14 in order to assess TPA toxicity.</li></br> | ||
<li style="text-align:left">Plating of BAP 1 bacteria on all solid media to assess TPA toxicity and the ability of TPA to be used as a carbon source.</br></br> | <li style="text-align:left">Plating of BAP 1 bacteria on all solid media to assess TPA toxicity and the ability of TPA to be used as a carbon source.</br></br> | ||
+ | </ol> | ||
<center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> | <center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> | ||
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<li style="text-align:left">Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase. </li> | <li style="text-align:left">Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase. </li> | ||
<li style="text-align:left">Gel migration on 0.8% agarose gel. </li></br> | <li style="text-align:left">Gel migration on 0.8% agarose gel. </li></br> | ||
− | + | </ol> | |
<center><strong><img src="https://static.igem.org/mediawiki/2015/9/91/IGEM_Pasteur-gel--week-10.png" alt="k" width="292" height="455" /></strong><u> </u><br /> | <center><strong><img src="https://static.igem.org/mediawiki/2015/9/91/IGEM_Pasteur-gel--week-10.png" alt="k" width="292" height="455" /></strong><u> </u><br /> | ||
<strong><u>Figure 8:</u></strong> Gel for verification of the ligation of BBa_K808030 and pDG011 plasmid.<br /> | <strong><u>Figure 8:</u></strong> Gel for verification of the ligation of BBa_K808030 and pDG011 plasmid.<br /> | ||
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</p> | </p> | ||
</br> | </br> | ||
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<center><h3><em>Gibson Assembly</em></h3></center> | <center><h3><em>Gibson Assembly</em></h3></center> | ||
</br> | </br> |
Revision as of 15:01, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-Esterase
Figure 8: Gel for verification of the ligation of BBa_K808030 and pDG011 plasmid. There is no band for the ligation sample. Gibson Assembly
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