Difference between revisions of "Team:Pasteur Paris/Week 11"
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<li style="text-align:left"> Bacterial growth of the bacteria in liquid medium (5 ml of LB + Cm 34 µg/ml) and on a Petri dish (LB + Cm 34 µg/ml) and overnight incubation at 37°C.</li></br> | <li style="text-align:left"> Bacterial growth of the bacteria in liquid medium (5 ml of LB + Cm 34 µg/ml) and on a Petri dish (LB + Cm 34 µg/ml) and overnight incubation at 37°C.</li></br> | ||
<li style="text-align:left">Storage at 4°C.</li></br> | <li style="text-align:left">Storage at 4°C.</li></br> | ||
− | <li style="text-align:left">Bacterial growth of the Bacteria in LB: incubation at 37°C for 1h30 at 140rpm.</li | + | <li style="text-align:left">Bacterial growth of the Bacteria in LB: incubation at 37°C for 1h30 at 140rpm.</li> |
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− | <li style="text-align:left"> | + | <li style="text-align:left"> 100 µl of bacteria in 5 ml of LB</li> |
− | + | <li style="text-align:left"> 100 µl of bacteria in 5 ml of LB + Cm 34 µg/ml</li> | |
− | + | <li style="text-align:left"> 5 ml of LB without bacteria as negative control</li> | |
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− | <li style="text-align:left">LB + | + | |
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<center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> | <center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> |
Revision as of 15:31, 18 September 2015
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Week 11 Yeast AssemblyThe Bacteria was first sent to the iGEM Toulouse and could have suffered from the travel so we ordered it again.
Enzymatic Assay of NB-Esterase
![]() Figure 8: Gel for verification of the ligation of BBa_K808030 and pDG011 plasmid. There is no band for the ligation sample. Gibson Assembly
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