Difference between revisions of "Team:Pasteur Paris/Week 14"
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<li style="text-align:left">After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.</li></br> | <li style="text-align:left">After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.</li></br> | ||
<li style="text-align:left">Assembly of cluster 1:</li></br> | <li style="text-align:left">Assembly of cluster 1:</li></br> | ||
− | <ul> | + | <ul style="text-align:left"> |
<li>Xba I and Pst I enzymatic digestion. </li> | <li>Xba I and Pst I enzymatic digestion. </li> | ||
<li>Gel migration on 1% agarose gel. </li> | <li>Gel migration on 1% agarose gel. </li> | ||
Line 149: | Line 149: | ||
<li>Gel Purification following the Macherey-Nagel protocol </li> | <li>Gel Purification following the Macherey-Nagel protocol </li> | ||
<li>DNA concentration assay using a spectrophotometer. </li> | <li>DNA concentration assay using a spectrophotometer. </li> | ||
+ | </ul> | ||
</ul> | </ul> | ||
</br> | </br> |
Revision as of 18:01, 18 September 2015
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Week 14
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