Difference between revisions of "Team:Pasteur Paris/Week 14"
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<li style="text-align:left">Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER </li></br> | <li style="text-align:left">Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER </li></br> | ||
<li style="text-align:left">After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.</li></br> | <li style="text-align:left">After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.</li></br> | ||
− | <li style="text-align:left">Assembly of cluster 1: | + | <li style="text-align:left">Assembly of cluster 1:</br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>Xba I and Pst I enzymatic digestion. </li> | <li>Xba I and Pst I enzymatic digestion. </li> | ||
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<li>DNA Concentration </li> | <li>DNA Concentration </li> | ||
</ul> | </ul> | ||
+ | </li> | ||
</br> | </br> | ||
<table border="1" cellspacing="0" cellpadding="0" width="604"> | <table border="1" cellspacing="0" cellpadding="0" width="604"> | ||
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</table> | </table> | ||
</br> | </br> | ||
− | |||
<li style="text-align:left">Midiprep of BBa_K808030: | <li style="text-align:left">Midiprep of BBa_K808030: | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
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</table> | </table> | ||
</br> | </br> | ||
− | |||
<li>Ligation of BBa_K808007 and BBa_K808030 </li> | <li>Ligation of BBa_K808007 and BBa_K808030 </li> | ||
<li>Transformation in electro-competent DH5-⍺ cells. </li> | <li>Transformation in electro-competent DH5-⍺ cells. </li> |
Revision as of 18:05, 18 September 2015
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Week 14
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