Difference between revisions of "Team:Pasteur Paris/Week 11"
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<li style="text-align:left">Electro-competent BAP 1 cells. </li></br> | <li style="text-align:left">Electro-competent BAP 1 cells. </li></br> | ||
<li style="text-align:left">Enzymatic digest of BBa_K808030 and pDG011 plasmid using XbaI and PstI. </li></br> | <li style="text-align:left">Enzymatic digest of BBa_K808030 and pDG011 plasmid using XbaI and PstI. </li></br> | ||
− | <li style="text-align:left">Transformation of pDG011 plasmid in chemically competent DH5- | + | <li style="text-align:left">Transformation of pDG011 plasmid in chemically competent DH5-α cells. </li></br> |
<li style="text-align:left">Digestion of BBa_K808030 and pDG011 plasmid with restriction enzyme XbaI and PstI. </li></br> | <li style="text-align:left">Digestion of BBa_K808030 and pDG011 plasmid with restriction enzyme XbaI and PstI. </li></br> | ||
<li style="text-align:left">Gel migration of BBa_K808030 and pDG011 plasmid. </li></br> | <li style="text-align:left">Gel migration of BBa_K808030 and pDG011 plasmid. </li></br> |
Revision as of 21:54, 18 September 2015
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Week 11 Yeast AssemblyThe Bacteria was first sent to the iGEM Toulouse and could have suffered from the travel so we ordered it again.
=> No bacterial growth was observed. Gibson Assembly
Figure 9: Gel of the migration of the PCR product with control.
Figure 10: Gel of the migration of the Biobricks BBa_K808007 and BBa_K808030.
=> No results on the gel.
Figure 11: Gel of the migration of the Biobricks BBa_K316003, BBa_K808014, BBa_K1392932, BBa_K936011, BBa_K195000, BBa_K1392931. pNP assay - Enzymatic assay
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