Difference between revisions of "Team:Czech Republic/Goals"

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==Goals of module 1==
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Build a synthetic haploids which are able of using the haploid specific yeast pheromone pathway after their mating.
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*Construct a set of reporter promoters for yeast cells
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*Characterize reporter promoters in all mating types
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*Design synthetic MATa and MATx strains
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*Build a synthetic diploid strain with a functional yeast pheromone pathway
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*Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids
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==Goals of module 2==
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* Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks
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* Verify the correct coupling of the receptors to the yeast pheromone mating pathway
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* Verify the correct expression and secretion of the different pheromones
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* Show the orthogonality of the used receptors and pheromones
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==Goals of module 3==
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* Demonstrate the ability of our receptors to bind chosen markers.
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Assemble as many sets of orthogonal pheromones and receptors as possible.
  
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Display selected proteins on the surface of the yeast.
  
 
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Revision as of 19:53, 18 September 2015

Goals

Build a synthetic haploids which are able of using the haploid specific yeast pheromone pathway after their mating.

Assemble as many sets of orthogonal pheromones and receptors as possible.

Display selected proteins on the surface of the yeast.