Difference between revisions of "Team:Pasteur Paris/Week 15"

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<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose cel.</br>
+
<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose gel.</br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4 :  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4 :  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
 
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells </li></br>
 
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells </li></br>
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<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931 : </p>
 
<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931 : </p>
 
<ul style="text-align: left">
 
<ul style="text-align: left">
   <li>Transformation in chemically competent <em>E. coli</em> DH5- cells. </li>
+
   <li>Transformation in chemically competent <em>E. coli</em> DH5-cells. </li>
 
</ul>
 
</ul>
  

Revision as of 21:31, 18 September 2015

Week 15

09/07 - 09/11




  1. Gibson Assembly of Cluster 4

  2. Figure xx: Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose cel.


  3. Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose gel.
  4. Gibson Assembly of cluster 4 : BBa_K936011, BBa_K936023 and pSB1C3.

  5. Transformation in chemically competent E. coli DH5-⍺ cells

  6. MiniPrep

  7. Preparation for sequencing.

  8. Stock of the plasmids containing BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K1392931 :

    • Transformation in chemically competent E. coli DH5-⍺ cells.


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