Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/6 July 2015"

Revision as of 00:20, 10 July 2015

Cell Lysis, continued

Calculations of Reagent Quantities Needed We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios: Lysozyme: 0.2 mg/mL of solution PMSF Solution: added to final concentration of 1mM Deoxycholic Acid: 1.5 g/g of cell pellet

Mass of Cell Pellet	Lysozyme	PMSF Solution	Deoxycholic Acid
0.52g	0.0082g	0.41mL	0.78g
0.32g	0.0082g	0.41mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.02g	0.0084g	0.42mL	0.03g
0.52g	0.0084g	0.42mL	0.78g
0.82g	0.0084g	0.42mL	1.23g
0.32g	0.0084g	0.42mL	0.48g
0.32g	0.0084g	0.42mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.22g	0.0084g	0.42mL	0.33g
0.52g	0.0084g	0.42mL	0.78g
0.62g	0.0084g	0.42mL	0.93g
0.42g	0.0084g	0.42mL	0.63g
0.32g	0.0084g	0.42mL	0.48g
0.12g	0.0084g	0.42mL	0.18g
0.62g	0.0084g	0.42mL	0.93g
0.52g	0.0084g	0.42mL	0.78g

@@ Line 288: / Line 288: @@
 <u>Cell Lysis, continued</u>
-Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)
+Calculations of Reagent Quantities Needed
+We account for inflation of cell pellet weight due to inclusion of Falcon tube mass, and the reagents are added in the following ratios:
+Lysozyme: 0.2 mg/mL of solution
+PMSF Solution: added to final concentration of 1mM
+Deoxycholic Acid: 1.5 g/g of cell pellet
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