Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/21 May 2015"
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| − | ='''Inoculation of 5/ | + | ='''Inoculation of 5/19 Transformation'''= |
*I picked four colonies from the 1:1 kanamycin plate and suspended each in 100uL ddH2O | *I picked four colonies from the 1:1 kanamycin plate and suspended each in 100uL ddH2O | ||
*Each sample was inoculated in 3 different culture tubes with 5mL LB broth and 5uL 1000X kanamycin and was incubated at 37C. | *Each sample was inoculated in 3 different culture tubes with 5mL LB broth and 5uL 1000X kanamycin and was incubated at 37C. | ||
Latest revision as of 21:15, 10 July 2015
Inoculation of 5/19 Transformation
- I picked four colonies from the 1:1 kanamycin plate and suspended each in 100uL ddH2O
- Each sample was inoculated in 3 different culture tubes with 5mL LB broth and 5uL 1000X kanamycin and was incubated at 37C.