Difference between revisions of "Team:UCLA/Notebook/Protein Cages/21 August 2015"
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OD with IPTG after two hours: 1.390 | OD with IPTG after two hours: 1.390 | ||
| − | + | Cells pelleted after three hours of induction at max speed for 20 min. Washed with sterile ddH2O. Repelleted. Frozen in -20C | |
| − | + | ||
| − | Cells pelleted at max speed for 20 min. Washed with sterile ddH2O. Repelleted. Frozen in -20C | + | |
Tyler Lee --[[User:Wtleeiv|Wtleeiv]] 01:24, 22 August 2015 (CDT) | Tyler Lee --[[User:Wtleeiv|Wtleeiv]] 01:24, 22 August 2015 (CDT) | ||
Revision as of 06:27, 22 August 2015
Intro: Grew the starter culture overnight for ~16 hours. Refreshed into 300 mL expression culture. Induced with IPTG.
Expression Culture:
OD with 4 mL of starter culture: 0.023
OD with all of starter culture: 0.049
Incubation with all of starter culture began at 1:25 pm.
OD after one hour: 0.073
OD after two hours: 0.112
OD after 3.5 hours: 0.443
OD after 4.5 hours: 1.091
Induced with IPTG to 1mM concentration at ~6:20pm.
OD with IPTG after one hour: 1.370
OD with IPTG after two hours: 1.390
Cells pelleted after three hours of induction at max speed for 20 min. Washed with sterile ddH2O. Repelleted. Frozen in -20C
Tyler Lee --Wtleeiv 01:24, 22 August 2015 (CDT)