Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/18 August 2015"

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='''Transformation with the Silk+GFP Construct'''=
 
='''Transformation with the Silk+GFP Construct'''=
  
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*Redo of the transformation.
 
*Using the new BL21 cells that I made last week
 
*Using the new BL21 cells that I made last week
  

Latest revision as of 21:56, 24 August 2015

Transformation with the Silk+GFP Construct

  • Redo of the transformation.
  • Using the new BL21 cells that I made last week
  1. Thaw cells (50uL) on ice for 10 minutes.
  2. Add 1uL of ligated product.
  3. Place on ice for 5 minutes.
  4. Rescue in 200uL of SOC, incubate and shake for 1 hour.
  5. Warm chloramphenicol plates at 37C.
  6. Spin down bacteria to pellet, discard supernatant.
  7. Make 2 serial dilutions (1:10 and 1:100)
  8. Plate 100uL of bacteria (1:1, 1:10, 1:100) on each plate.
  9. Spread with beads, incubate at 37C.