Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/18 May 2015"
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*Growing starter culture from grown Tamura insert pSB1A3 vector in NEB E. coli DH5alpha cells. | *Growing starter culture from grown Tamura insert pSB1A3 vector in NEB E. coli DH5alpha cells. | ||
**Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony. | **Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony. | ||
| + | [[File:UCLA iGEM Tamura 05 Plate 5-18.jpg|200px|thumb|left|Transformed colonies from Tamura insert in pSB1A3 backbone. Grown using NEB E. coli K12 DH5alpha strains.]] | ||
Revision as of 01:56, 19 May 2015
- Growing starter culture from grown Tamura insert pSB1A3 vector in NEB E. coli DH5alpha cells.
- Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony.
