Difference between revisions of "Team:Vilnius-Lithuania/Methods"

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                         <h4 class="panel-title">1. Plasmid DNA extraction</h4>
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                            Name
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                            Protocol
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  <strong>Materials:</strong><br />
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  4 mL of LB medium<br />
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  Antibiotic<br />
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  Transformed bacteria<br />
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  <strong>Process</strong>:<br />
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  Transformed bacteria, which contain a plasmid with a target DNA, is placed into laboratory tube with 4 ml of liquid LB  medium and specific antibiotic. Bacteria are then placed into the shaker during the night at 37ºC. For the plasmid DNA  extraction we used GeneJet Plasmid Miniprep Kit, according to manufacterer's instructions (pdf file).
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Revision as of 23:11, 1 September 2015

Materials:
4 mL of LB medium
Antibiotic
Transformed bacteria
Process:
Transformed bacteria, which contain a plasmid with a target DNA, is placed into laboratory tube with 4 ml of liquid LB medium and specific antibiotic. Bacteria are then placed into the shaker during the night at 37ºC. For the plasmid DNA extraction we used GeneJet Plasmid Miniprep Kit, according to manufacterer's instructions (pdf file).