Difference between revisions of "Team:Czech Republic/Project/Synthetic haploids"
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== Design == | == Design == | ||
Revision as of 14:18, 6 September 2015
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Contents
Protocols page:
Intro / Background
Test [Janiak2005].
Overview
Key Achievements
PUT THIS ELSEWHERE WIKI PEOPLE
- Hello
Design
Concept
Signals
TODO: Scheme of the MF(ALPHA)1 - Benchling?
DNA
- Genomic PCR of WT SC-STE2 (YFL026W - http://www.yeastgenome.org/locus/S000001868/overview) - ORF
- Genomic PCR of MF(ALPHA)1 (YPL187W - http://www.yeastgenome.org/locus/mf%28alpha%291/overview) - secretion tag (first PCR), second PCR to add the actual pheromone and stop codon
Materials and methods
Chemicals and strains
Construction
Validation
Results
Final constructs
Proof of concept test
Test of mating types
References
- ↑ Lin, C.-H., Choi, a., & Bennett, R. J. (2011). Defining pheromone-receptor signaling in Candida albicans and related asexual Candida species. Molecular Biology of the Cell, 22(24), 4918–4930. doi:10.1091/mbc.E11-09-0749