Difference between revisions of "Team:Czech Republic/Notebook"
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=== Tuesday === | === Tuesday === | ||
| − | * [[--|Gel electrophoresis]] - pRSII416 | + | * [[--|Gel electrophoresis]] - pRSII416 from this Monday |
| − | * [[--|Gel purification]] - | + | * [[--|Gel purification]] - extraction and purification of the digested pRSII416 vector |
* [[--|Ligation]] - | * [[--|Ligation]] - | ||
* [[--|Transformation]] - | * [[--|Transformation]] - | ||
Revision as of 09:04, 7 September 2015
Contents
Notebook
May
Week 1 (4-10)
Module 2
Monday
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain.
- Genome extraction
- PCR - annealing temperature was 55°C, 30 cycles
- Gel electrophoresis - verification of successfull amplification of ste2
- Restriction digest - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
Tuesday
- Gel electrophoresis - pRSII416 from this Monday
- Gel purification - extraction and purification of the digested pRSII416 vector
- Ligation -
- Transformation -
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September