Difference between revisions of "Team:Czech Republic/Notebook"
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* [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | * [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | ||
| − | === Tuesday === | + | ==== Tuesday ==== |
* [[--|Gel electrophoresis]] - pRSII416 from this Monday | * [[--|Gel electrophoresis]] - pRSII416 from this Monday | ||
* [[--|Gel purification]] - extraction and purification of the digested pRSII416 vector | * [[--|Gel purification]] - extraction and purification of the digested pRSII416 vector | ||
* [[--|Ligation]] - 26.4ng of the insert | * [[--|Ligation]] - 26.4ng of the insert | ||
* [[--|Transformation]] - 5μL of the ligation was transformed into E5alpha cells. After 2h of incubation at 37°C, 300μL was spread on Amp plate. | * [[--|Transformation]] - 5μL of the ligation was transformed into E5alpha cells. After 2h of incubation at 37°C, 300μL was spread on Amp plate. | ||
| + | |||
| + | ==== Wednesday ==== | ||
== Week 2 (11-17) == | == Week 2 (11-17) == | ||
Revision as of 16:43, 7 September 2015
Contents
Notebook
May
Week 1 (4-10)
Module 2
Monday
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain.
- Genome extraction
- PCR - annealing temperature was 55°C, 30 cycles
- Gel electrophoresis - verification of successfull amplification of ste2
1.3kb
- Restriction digest - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
Tuesday
- Gel electrophoresis - pRSII416 from this Monday
- Gel purification - extraction and purification of the digested pRSII416 vector
- Ligation - 26.4ng of the insert
- Transformation - 5μL of the ligation was transformed into E5alpha cells. After 2h of incubation at 37°C, 300μL was spread on Amp plate.
Wednesday
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September