Difference between revisions of "Team:Czech Republic/Notebook"

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== Week 1 (4-10) ==
 
== Week 1 (4-10) ==
 
=== Module 2 ===
 
=== Module 2 ===
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==== Monday ====
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PCR of the ste2 gene from the genome of ''Saccharomyces cerevisiae'' 7284 strain ([[--|Genome extraction]], [[--|PCR]])
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PCR of the ste2 gene from the genome of ''Saccharomyces cerevisiae'' 7284 strain.
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[[--|Gel electrophoresis]] - verification of successfull amplification of ste2
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* [[--|Genome extraction]]
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* [[--|PCR]] - annealing temperature was 55°C, 30 cycles
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* [[--|Gel electrophoresis]] - verification of successfull amplification of ste2
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[[File:Czech_Republic_M2_gel-05-04.png|250px]]
 
[[File:Czech_Republic_M2_gel-05-04.png|250px]]
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1.3kb
 
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* [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
 
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==== Tuesday ====
 
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* [[--|Gel electrophoresis]] - pRSII416 from this Monday
 
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* [[--|Gel purification]] - extraction and purification of the digested pRSII416 vector
 
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* [[--|Ligation]] - 26.4ng of the insert
 
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* [[--|Transformation]] - 5μL of the ligation was transformed into E5alpha cells. After 2h of incubation at 37°C, 300μL was spread on Amp plate.
 
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==== Wednesday ====
 
  
 
== Week 2 (11-17) ==
 
== Week 2 (11-17) ==

Revision as of 06:39, 10 September 2015

Notebook

May

Week 1 (4-10)

Module 2

PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain (Genome extraction, PCR) Gel electrophoresis - verification of successfull amplification of ste2 Czech Republic M2 gel-05-04.png

Week 2 (11-17)

Week 3 (18-24)

Week 4 (25-31)

June

July

August

September