Difference between revisions of "Team:Czech Republic/Notebook"
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| − | Cloning of the amplified ste2 into pRSII416 vector. ([[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Ligation|Ligation]], [[Team:Czech_Republic/Protocols#Transformation_(bacteria)|Transformation]]) | + | Cloning of the amplified ste2 into pRSII416 vector. ([[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Ligation|Ligation]], [[Team:Czech_Republic/Protocols#Transformation_(bacteria)|Transformation]]). |
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| + | Verification of the pRSII416_ste2 construct ([[Team:Czech_Republic/Protocols#Miniprep|Miniprep]], [[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Gel_electrophoresis|Gel electrophoresis]]). | ||
== Week 2 (11-17) == | == Week 2 (11-17) == | ||
Revision as of 07:15, 10 September 2015
Notebook
Contents
May
Week 1 (4-10)
Module 2
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain (Genome extraction, PCR, Gel electrophoresis).
Cloning of the amplified ste2 into pRSII416 vector. (Restriction digest, Ligation, Transformation).
Verification of the pRSII416_ste2 construct (Miniprep, Restriction digest, Gel electrophoresis).
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September