Difference between revisions of "Team:Czech Republic/Microfluidics"
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Microfluidic channels were formed using PDMS soft-lithography technology, which has proven biocompatibility and can be readily applied in available laminar flow cabinets (photomask and silicon master fabrication is outsourced). Silicon masters were required for the PDMS molding. PDMS molds were bonded to the glass substrates to form encapsulated microfluidic devices using air plasma technology. | Microfluidic channels were formed using PDMS soft-lithography technology, which has proven biocompatibility and can be readily applied in available laminar flow cabinets (photomask and silicon master fabrication is outsourced). Silicon masters were required for the PDMS molding. PDMS molds were bonded to the glass substrates to form encapsulated microfluidic devices using air plasma technology. | ||
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| + | Two part silicone elastomer Sylgard 184 is used to produce PDMS. The base part is mixed with sufficient amount of curing agent (10:1 ratio) and stirred well in a disposable plastic cup. The mixture is placed in dessicator to remove the air bubbles introduced by the mixing. The Si master is placed in a petri dish and the mixture is poured over. The remaining air bubbles are removed from the PDMS by sharp tip of a needle. The poured PDMS is maintained in perfect horizontal position to assure good planarity, and is cured in an oven, for 2 hours at 80◦C. The PDMS edges are cut off with sharp tool and the PDMS is peeled off the Si master. The PDMS mold is sliced into sections containing individual devices. Inlets and outlets are drilled carefully by biopsy punch of the appropriate diameter at the desired locations of the PDMS replica. | ||
[[File:SoftLitho.jpg|thumbnail|Soft-lithography process workflow]] | [[File:SoftLitho.jpg|thumbnail|Soft-lithography process workflow]] | ||
Revision as of 11:30, 10 September 2015
Microfluidics
Contents
Introduction
Microfluidic technology showed different perspective of the fluid and cell handling. The diffusion processes are slow, and the inertial effects are negligible on micro-scale with low Reynolds number [6,55]. It became a valuable tool enabling complex control of the cellular microenvironment.
Soft lithography description [ Fikar2015].
Soft-lithography
Microfluidic channels were formed using PDMS soft-lithography technology, which has proven biocompatibility and can be readily applied in available laminar flow cabinets (photomask and silicon master fabrication is outsourced). Silicon masters were required for the PDMS molding. PDMS molds were bonded to the glass substrates to form encapsulated microfluidic devices using air plasma technology.
Two part silicone elastomer Sylgard 184 is used to produce PDMS. The base part is mixed with sufficient amount of curing agent (10:1 ratio) and stirred well in a disposable plastic cup. The mixture is placed in dessicator to remove the air bubbles introduced by the mixing. The Si master is placed in a petri dish and the mixture is poured over. The remaining air bubbles are removed from the PDMS by sharp tip of a needle. The poured PDMS is maintained in perfect horizontal position to assure good planarity, and is cured in an oven, for 2 hours at 80◦C. The PDMS edges are cut off with sharp tool and the PDMS is peeled off the Si master. The PDMS mold is sliced into sections containing individual devices. Inlets and outlets are drilled carefully by biopsy punch of the appropriate diameter at the desired locations of the PDMS replica.
Experimental setup
Microfluidic experiments were conducted on a platform already established by the Georgiev lab. The laboratory is equipped with precise microfluidic syringe system (neMESYS Low Pressure Syringe Pumps), and microscopic station enabling fluorescence imaging and live cell microscopy (Olympus IX83).
Appendix
Personnel
Martin Cienciala - Responsible person
Pavel Fikar - Scientific advisor
References
- ↑ Lin, C.-H., Choi, a., & Bennett, R. J. (2011). Defining pheromone-receptor signaling in Candida albicans and related asexual Candida species. Molecular Biology of the Cell, 22(24), 4918–4930. doi:10.1091/mbc.E11-09-0749