Difference between revisions of "Team:Vilnius-Lithuania/Measurement"

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       <div class="caption" style="color: rgb(00,00,00); background-color: rgb(249,249,249);">
 
       <div class="caption" style="color: rgb(00,00,00); background-color: rgb(249,249,249);">
 
         <h3 class="text-heading">Description</h3>
 
         <h3 class="text-heading">Description</h3>
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           <p class="text-center"><em>Under construction.</em></p>
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           <p class="text-justify">This year Vilnius iGEM team is also participating in the iGEM Measurement InterLab study. This is the second year that iGEM holds an InterLab study. This is one massive amazing experiment which involves multiple teams all around the world working for the same scientific goal. </p>
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          <p class="text-justify" style="height: auto; clear: both; margin: auto; padding-bottom: 20px;">This year the aim of this study is to see how different laboratories perform the same experiment and how results between different laboratories can differ or resemble each other. The ultimate goal of InterLab study is to develop new methods of interpreting data, which would take into account the differences that might arise whilst performing similar experiments in different conditions.</p>
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          <p class="text-justify">All teams had to construct three different GFP expressing devices and subsequently measure their fluorescence output. Received fluorescence data reflects the strength of gene (GFP) expression.</p>
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          <p class="text-justify">Three devices had to be constructed:</p>
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          <ol>
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<li> <a href="http://parts.igem.org/Part:BBa_J23101" style="color:darkgreen">J23101</a> + <a href="http://parts.igem.org/Part:BBa_I13504" style="color:darkgreen">I13504</a></li>
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<li> <a href="http://parts.igem.org/Part:BBa_J23106" style="color:darkgreen">J23106</a> + <a href="http://parts.igem.org/Part:BBa_I13504" style="color:darkgreen">I13504</a></li>
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<li> <a href="http://parts.igem.org/Part:BBa_J23117" style="color:darkgreen">J23117</a> + <a href="http://parts.igem.org/Part:BBa_I13504" style="color:darkgreen">I13504</a></li>
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          </ol>
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          <p class="text-justify">J231xx parts are different promoters characteristic of different transcription levels. I13504 is ribosome binding sequence (RBS) + GFP + two terminators.</p>
 
       </div>
 
       </div>
 
     </div>
 
     </div>

Revision as of 09:20, 12 September 2015

Description

This year Vilnius iGEM team is also participating in the iGEM Measurement InterLab study. This is the second year that iGEM holds an InterLab study. This is one massive amazing experiment which involves multiple teams all around the world working for the same scientific goal.

This year the aim of this study is to see how different laboratories perform the same experiment and how results between different laboratories can differ or resemble each other. The ultimate goal of InterLab study is to develop new methods of interpreting data, which would take into account the differences that might arise whilst performing similar experiments in different conditions.

All teams had to construct three different GFP expressing devices and subsequently measure their fluorescence output. Received fluorescence data reflects the strength of gene (GFP) expression.

Three devices had to be constructed:

  1. J23101 + I13504
  2. J23106 + I13504
  3. J23117 + I13504

J231xx parts are different promoters characteristic of different transcription levels. I13504 is ribosome binding sequence (RBS) + GFP + two terminators.

Experiments

Under construction.

Results

Under construction.

Discussion

Under construction.

References

  • 1
  • 2
  • 3
  • 4