Difference between revisions of "Team:Vilnius-Lithuania/Measurement"
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<div class="caption" style="color: rgb(00,00,00); background-color: rgb(249,249,249);"> | <div class="caption" style="color: rgb(00,00,00); background-color: rgb(249,249,249);"> | ||
<h3 class="text-heading">Experiments</h3> | <h3 class="text-heading">Experiments</h3> | ||
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<h3 style="border-left: 5px solid rgb(236,151,31); padding-left: 5px; border-bottom: none">Cloning</h3> | <h3 style="border-left: 5px solid rgb(236,151,31); padding-left: 5px; border-bottom: none">Cloning</h3> | ||
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<p class="text-justify">All three constructs were grown as three biological replicates. We used positive control – a GFP expressing device – <a href="http://parts.igem.org/Part:BBa_I20270" style="color:darkgreen">BBa_I20270</a>. We also used a negative control, which did not have the GFP gene in it – <a href="http://parts.igem.org/Part:BBa_R0040" style="color:darkgreen">BBa_R0040</a>. The positive and negative controls were grown in biological triplicates. All bacteria were grown in a LB medium overnight.</p> | <p class="text-justify">All three constructs were grown as three biological replicates. We used positive control – a GFP expressing device – <a href="http://parts.igem.org/Part:BBa_I20270" style="color:darkgreen">BBa_I20270</a>. We also used a negative control, which did not have the GFP gene in it – <a href="http://parts.igem.org/Part:BBa_R0040" style="color:darkgreen">BBa_R0040</a>. The positive and negative controls were grown in biological triplicates. All bacteria were grown in a LB medium overnight.</p> | ||
+ | <h3 style="border-left: 5px solid rgb(236,151,31); padding-left: 5px; border-bottom: none">OD measurements</h3> | ||
+ | <p class="text-justify">Optical density (OD) of bacteria grown overnight was measured and subsequently diluted to an OD<sub>600</sub> value of 0.5.</p> | ||
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+ | <h3 style="border-left: 5px solid rgb(236,151,31); padding-left: 5px; border-bottom: none">Fluorescence measurements</h3> | ||
+ | <p class="text-justify">After dilutions to OD<sub>600</sub>= 0.5, bacteria were measured for their fluorescence in a Jobin Ivon HORIBA FluoroMax-3 spectrofluorometer. The excitation was carried out at 500 nm and emission was recorded at 511 nm.</p> | ||
</div> | </div> |
Revision as of 10:04, 12 September 2015