Difference between revisions of "Team:Pasteur Paris/Week 1"

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<p>1/ Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007,  BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000,  BBa_K316003.<br>
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  </p>
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<li style="text-align:left">Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007,  BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000,  BBa_K316003.</li><br>
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<p>2/ Storage at -20°C.</p>
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<li style="text-align:left">Storage at -20°C.</li>
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<p>3/ Transformation in DH5α Competent cells  purchased from Invitrogen (# 18265-017) was done following the transformation  protocol. (BBa_K808007, BBa_K808010, BBa_K808011,  BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).</p>
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<li style="text-align:left">Transformation in DH5α Competent cells  purchased from Invitrogen (# 18265-017) was done following the transformation  protocol. (BBa_K808007, BBa_K808010, BBa_K808011,  BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).</li>
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<p>4/ Cells were plated on LB + chloramphenicol 34µg/mL (Cm34). </p>
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<li style="text-align:left">Cells were plated on LB + chloramphenicol 34µg/mL (Cm34). </li>
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<p>5/ Incubation  overnight. </p>
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<li style="text-align:left">5/ Incubation  overnight. </li>
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<p>6/ Storage at 4°C. </p>
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<li style="text-align:left">6/ Storage at 4°C. </li>
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<p>7/ Plasmid  purification following the MiniPrep protocol. </p>
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<li style="text-align:left">7/ Plasmid  purification following the MiniPrep protocol. </li>
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Revision as of 00:41, 13 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 1

06/01 - 06/05

  1. Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Storage at -20°C.
  3. Transformation in DH5α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).
  4. Cells were plated on LB + chloramphenicol 34µg/mL (Cm34).
  5. 5/ Incubation overnight.
  6. 6/ Storage at 4°C.
  7. 7/ Plasmid purification following the MiniPrep protocol.

BBa

Concentration (ng/µL)

Clone 1

Clone 2

Clone 3

808007

73.4

44.3

56.3

808010

40.8

27.5

29.5

808011

28.8

30.4

18.5

808012

49.5

#VALEUR!

56.5

808013

18.8

26

15.7

808014

30

41.4

37.4

316003

51.5

23.5

36.1

936000

15.2

17.7

13.8

 



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